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Eukaryotic Cell Cultures pp 405–418Cite as

Cultured Human T Lymphocyte Lines and Clones as Immunogenetic Tools

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

Abstract

Human T lymphocytes were sensitized in vitro to allogeneic determinants encoded by the HLA region and subsequently cloned by limiting dilution in the presence of human T cell growth factor. An in vitro priming protocol entailing multiple rounds of allostimulation in mixed lymphocyte culture, using a disparate stimulating to responding cell ratio (10:1), enabled a high frequency of functionally active clones to be isolated. The specificity of the proliferative and cytotoxic reactivities was ascertained directly by screening in an HLA characterized family.

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Author information

Authors and Affiliations

  1. Institute of Immunology, University of Munich Munich, Federal Republic of Germany

    Dolores J. Schendel & Rudolf Wank

Authors
  1. Dolores J. Schendel
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  2. Rudolf Wank
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Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    Ronald T. Acton  & J. Daniel Lynn  & 

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© 1984 Plenum Press, New York

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Schendel, D.J., Wank, R. (1984). Cultured Human T Lymphocyte Lines and Clones as Immunogenetic Tools. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_23

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  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_23

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-9378-2

  • Online ISBN: 978-1-4615-9376-8

  • eBook Packages: Springer Book Archive

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