Skip to main content
SpringerLink
Log in

Alloreactive T Cell Clones

  • Chapter
  • First Online:
Eukaryotic Cell Cultures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

Abstract

T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non-cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly the characteristics of the resulting cells. The method of choice depends on the specific question to be asked.

This research was supported by USPHS Grants AI-04197, AI-16558, and CA-19226.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

BCSF:

B cell stimulating factor

CM:

conditioned medium

CSF:

colony stimulating factor

CTL:

cytolytic T lymphocytes

IL-2:

interleukin 2

IL-3:

interleukin 3

MHC:

major histocompatibility complex

MLC:

mixed leukocyte culture

MoLV:

Moloney leukemia virus

References

  1. Morgan, D.A., Ruscetti, F.W. and Gallo, R. 1976. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science 193:1007.

    Article  Google Scholar 

  2. Gordon, J. and MacLean, L.D. 1965. A lymphocyte-stimulating factor produced in vitro. Nature 208:795.

    Article  Google Scholar 

  3. Kasakura, S. and Lowenstein, L. 1965. A factor stimulating DNA synthesis derived from the medium of leucocyte cultures. Nature 208:794.

    Article  Google Scholar 

  4. Gillis, S. and Smith, K.A. 1977. Long term culture of tumor-specific cytotoxic T cells. Nature 268:154.

    Article  Google Scholar 

  5. Nabholz, M., Engers, H.D., Collavo, D. and North, M. 1978. Cloned T-cell lines with specific cytolytic activity. Curr. Top. Microbiol. Immunol. 81:176.

    Google Scholar 

  6. Haas, W., Mathur-Rochat, J., Pohlit, H., Nabholz, M. and von Boehmer, H. 1980. Cytotoxic T cell responses to haptenated cells. III. Isolation and specificity analysis of continuously growing clones. Eur. J. Immunol. 10:828.

    Article  Google Scholar 

  7. von Boehmer, H. and Haas, W. 1981. H-2 restricted cytolytic and noncytolytic T cell clones: Isolation, specificity and functional analysis. Immunol. Rev. 54:27.

    Article  Google Scholar 

  8. Engers, H.D., Collavo, D. North, M., von Boehmer, H., Haas, W., Hengartner, H. and Nabholz, M. 1980. Characterization of cloned murine cytolytic T cells lines. J. Immunol. 125:1481.

    Google Scholar 

  9. Hengartner, H. and Fathman, C.G. 1980. Clones of alloreactive T cells. I. A unique homozygous MLR-stimulating determinant present on B6 stimulators. Immunogenetics 10:175.

    Article  Google Scholar 

  10. Glasebrook, A.L., Sarmiento, M., Loken, M.R., Dialynas, D.P., Quintans, J., Eisenberg, L., Lutz, C.T., Wilde, D. and Fitch, F.W. 1981. Murine T lymphocyte clones with distinct immunological functions. Immunol. Rev. 54:225.

    Article  Google Scholar 

  11. MacDonald, H.R., Cerottini, J.-C., Ryser, J.-E., Maryanski, J.L., Taswell, C., Widmer, M.B. and Brunner, K.T. 1980. Quantitation and cloning of cytolytic T lymphocytes and their precursors. Immunol. Rev. 51:93.

    Article  Google Scholar 

  12. Harwell, L., Skidmore, B., Marrack, P. and Kappler J. 1980. Concanavalin A-inducible, interleukin-2-producing T cell hybridoma. J. Exp. Med. 152:893.

    Article  Google Scholar 

  13. Nabholz, M., Cianfriglia, M., Acuto, O. Conzelman, A., Weiss, A., Haas, W. and von Boehmer, H. 1980. Cytolytically active murine T-cell hybrids. Nature 287:437.

    Article  Google Scholar 

  14. Taniguchi, M., Saito, T. and Tada, T. 1979. Antigen-specific suppressive factor produced by a transplantable I-J bearing T-cell hybridoma. Nature 278:555.

    Article  Google Scholar 

  15. Farrar, J.J., Fuller-Farrar, J., Simon, P.L., Hilfiker, M.L., Stadler, B.M. and Farrar, W.L. 1980. Thymoma production of T cell growth factor (Interleukin 2). J. Immunol. 125:2555.

    Google Scholar 

  16. Shimizu, S., Kanaka, Y. and Smith, R. 1980. Mitogen-induced synthesis and secretion of T cell growth factors by a T lymphoma line. J. Exp. Med. 152:1436.

    Article  Google Scholar 

  17. Taussig, M., Holliman, A. and Wright, L.J. 1980. Hybridization between T and B lymphoma cell lines. Immunology 39:57.

    Google Scholar 

  18. Baker, P.E., Gillis, S. and Smith, K.A. 1979. Monoclonal cytolytic T-cell lines. J. Exp. Med. 149:273.

    Article  Google Scholar 

  19. Kimoto, M. and Fathman, C.G. 1980. Antigen-reactive T cell clones. I. Transcomplementing hybrid I-A-region gene products function effectively in antigen presentation. J. Exp. Med. 152:759.

    Article  Google Scholar 

  20. Sredni, B. and Schwartz, R.H. 1980. Alloreactivity of an antigen-specific T-cell clone. Nature 287:855.

    Article  Google Scholar 

  21. Fathman, C.G. and Hengartner, H. 1978. Clones of alloreactive T cells. Nature 272:617.

    Article  Google Scholar 

  22. Sredni, B., Tse, H.Y. and Schwartz, R.H. 1980. Direct cloning and extended cultures of antigen-specific MHC-restricted, proliferating T lymphocytes. Nature 283:581.

    Article  Google Scholar 

  23. von Boehmer, H., Hengartner, H., Nabholz, M., Lernhardt, W., Schreier, M. and Haas, W. 1979. Fine specificity of a continuously growing killer cell clone specific for H-Y antigen. Eur. J. Immunol. 9:592.

    Article  Google Scholar 

  24. Glasebrook, A.L. and Fitch, F.W. 1980. Alloreactive cloned T cell lines. I. Interactions between cloned amplifier and cytolytic T cell lines. J. Exp. Med. 151:876.

    Article  Google Scholar 

  25. Weiss, A., Brunner, K.T., MacDonald, H.R. and Cerottini, J.-C. 1980. Antigenic specificity of the cytolytic T lymphocyte response to murine sarcoma virus-induced tumors. III. Characterization of cytolytic T lymphocyte clones specific for Moloney leukemia virus-associated cell surface antigens. J. Exp. Med. 152:1210.

    Article  Google Scholar 

  26. Taswell, C. 1981. Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis. J. Immunol. 126:1614.

    Google Scholar 

  27. MacDonald, H.R., Maryanski, J.L. and Cerottini, J.-C. 1980. Cloning of cytolytic T lymphocytes: requirement for Interleukin 2 and irradiated spleen cells. Behring Inst. Mitt. 67:182.

    Google Scholar 

  28. Widmer, M.B. and Bach, F.H. 1981. Antigen driven helper cell-independent cloned cytolytic T lymphocytes. Nature 294:750.

    Article  Google Scholar 

  29. Glasebrook, A.L. 1982. Cytolytic T cell clones which proliferate autonomously to specific alloantigenic stimulation. Frequency Interleukin-2 production and Lyt phenotype. (Submitted for publication.

    Google Scholar 

  30. Glasebrook, A.L. and Fitch, F.W. 1979. T-cell lines which cooperate in generation of specific cytolytic activity. Nature 278:171.

    Article  Google Scholar 

  31. Sherman, L. 1980. Dissection of the B10.D2 anti-H-2Kb cytolytic T lymphocyte receptor repertoire. J. Exp. Med. 151:1386.

    Article  Google Scholar 

  32. Zinkernagel, R.M. and Doherty, P.C. 1979. MHC-cytotoxic T cells: Studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction specificity, function and responsiveness. Adv. Immunol. 27:51.

    Article  Google Scholar 

  33. Weiss, A., MacDonald, H.R., Cerottinin, J.-C. and Brunner, K.T. 1981. Inhibition of cytolytic T lymphocyte clones reactive with Moloney leukemia virus-associated antigens by monoclonal antibodies: a direct approach to the study of H-2 restriction. J. Immunol. 126:482.

    Google Scholar 

  34. Braciale, T.J., Andrew, M.E. and Braciale, V.L. 1981. Simultaneous expression of H-2 restricted and alloreactive recognition by a cloned line of influenza virus-specific cytotoxic T lymphocytes. J. Exp. Med. 153:1371.

    Article  Google Scholar 

  35. Braciale, T.J., Andres, M.E. and Braciale, V.L. 1981. Heterogeneity and specificity of cloned lines of influenza-virus specific cytotoxic T lymphocytes. J. Exp. Med. 153:910.

    Article  Google Scholar 

  36. Fathman, C.G. and Kimoto, M. 1980. Studies utilizing murine T cell clones: Ir genes, Ia antigens and MLR stimulating determinants. Immunol. Rev. 54:57.

    Article  Google Scholar 

  37. Fathman, C.G. and Hengartner, H. 1979. Crossreactive mixed lymphocyte reaction determinants recognized by cloned alloreactive T cells. Proc. Natl. Acad. Sci. USA 76:5863.

    Article  Google Scholar 

  38. Fathman, C.G., Kimoto, M., Melvold, R. and David, C.S. 1981. Reconstruction of Ir genes, Ia antigens, and mixed leukocyte reaction determinants by gene complementation. Proc. Natl. Acad. Sci. USA 78:1853.

    Article  Google Scholar 

  39. Sredni, B., Matis, L.A., Lerner, E.A., Paul, W.E. and Schwartz, R.H. 1981. Antigen-specific T cell clones restricted to unique F1 major histocompatibility complex determinants. Inhibition of proliferation with a monoclonal anti-Ia antibody. J. Exp. Med. 153:677.

    Article  Google Scholar 

  40. Sredni, B. and Schwartz, R.H. 1981. Antigen-specific, proliferating T lymphocyte clones. Methodology, specificity, MHC restriction, and alloreactivity. Immunol. Rev. 54:186.

    Article  Google Scholar 

  41. Webb, S.R., Molnar-Kimber, K.L., Bruce, J., Sprent, J. and Wilson, D.B. 1982. T cell clones with dual specificity for Mls and various major histocompatible complex determinants. J. Exp. Med. 154:1970.

    Article  Google Scholar 

  42. Schreier, M.H., Iscover, N.N., Teese, R., Aarden, L. and von Boehmer, H. 1980. Clones of killer and helper T cells: growth requirements, specificity and retention of function in long-term culture. Immunol. Rev. 51:315.

    Article  Google Scholar 

  43. Ely, J.M., Prystowsky, M.B., Eisenberg, L., Quintans, J., Goldwasser, E. and Fitch, F.W. 1981. Alloreactive cloned T cell lines. IV. Differential kinetics of IL-2, CSF, and BCSF release by a cloned T amplifier cell and its variant. J. Immunol. 127:2345.

    Google Scholar 

  44. Nabel, G., Greenberger, J.S., Sakakeeny, M.A. and Cantor, H. 1981. Multiple biologic activities of a cloned inducer T-cell population. Proc. Natl. Acad. Sci. USA 78:1157.

    Article  Google Scholar 

  45. Prystowsky, M.D., Ely, J.M., Beller, D.I., Eisenberg, L., Goldman, M., Goldman, J., Ihle, J., Quintans, J., Remold, H., Vogel, S.N., Goldwasser, E. and Fitch F.W. 1981. Alloreactive cloned T cell lines. VI. Multiple lymphokine activities secreted by helper and cytolytic cloned T lymphocytes. J. Immunol. 129:2337.

    Google Scholar 

  46. Glasebrook, A.L., Quintans, J., Eisenberg, L. and Fitch, F.W. 1981. Alloreactive cloned T cell lines. II. Polyclonal stimulation of B cells by a cloned helper T cell line. J. Immunol. 126:240.

    Google Scholar 

  47. Marcucci, F., Waller, M., Kirchner, H. and Krammer, P. 1981. Production of immune interferon by murine T-cell clones from long-term cultures. Nature 291:79.

    Article  Google Scholar 

  48. Fresno, M., Nabel, G., McVay-Bourdreau, L., Furthmayer, H. and Cantor, H. 1981. Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity. J. Exp. Med. 153:1246.

    Article  Google Scholar 

  49. Glasebrook, A.L., Kelso, A., Zubler, R.H., Ely, J.M., Prystowsky, M.B. and Fitch, F.W. 1982. Lymphokine production by cytolytic and noncytolytic alloreactive T cell clones. In: Isolation, Characterization and Utilization of T Lymphocyte Clones. (Fathman, C.G. and Fitch, F.W., eds.) Academic Press, Inc. New York, p. 342.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Committee on Immunology, The University of Chicago, Chicago, Illinois, USA

    Frank W. Fitch

Authors
  1. Frank W. Fitch
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    Ronald T. Acton  & J. Daniel Lynn  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Plenum Press, New York

About this chapter

Cite this chapter

Fitch, F.W. (1984). Alloreactive T Cell Clones. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_20

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-9378-2

  • Online ISBN: 978-1-4615-9376-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation