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Cloning of the Rat Endogenous Helper Leukemia Virus DNA Sequence and Expression of the Helper Activity Encoded by the Cloned DNA Sequence in Normal Rat Kidney Cells by Microinjection

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Eukaryotic Cell Cultures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

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Abstract

By the use of recombinant DNA technology and microinjection in cultured cells, the molecular genetic elements involved in the evolution of a retrovirus with the multipotential to infect, transform and replicate in host cell, have been critically examined in this investigation.

WR-RaLV: wild rat leukemia virus, RHHV: rat hepatoma helper virus, HTC-H1: hepatoma tissue culture cell, line H1, LTR: long terminal repeat polynucleotide sequence, Src: sarcomagenic DNA sequence, K-NRK: Kirsten sarcoma virus transformed non-producer, FCS: fetal calf serum, kb: kilobase, bp: base pair, KSV(RHHV): Kirsten sarcoma virus rat hepatoma helper virus pseudotype, TEN: Tris-HCl, 0.01 M, pH 7.5, EDTA, 0.001 M, NaCl, 0.15 M.

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Authors and Affiliations

  1. Laboratory of Cell Biology, National Cancer Institute, Bethesda, Maryland, 20205, USA

    Stringer S. Yang & Rama Modali

  2. Department of Tumor Virology, University of Texas System, Houston, Texas, 77030, USA

    Edwin Murphy Jr.

Authors
  1. Stringer S. Yang
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  2. Rama Modali
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  3. Edwin Murphy Jr.
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Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    Ronald T. Acton  & J. Daniel Lynn  & 

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© 1984 Plenum Press, New York

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Yang, S.S., Modali, R., Murphy, E. (1984). Cloning of the Rat Endogenous Helper Leukemia Virus DNA Sequence and Expression of the Helper Activity Encoded by the Cloned DNA Sequence in Normal Rat Kidney Cells by Microinjection. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_18

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  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_18

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