Skip to main content
SpringerLink
Log in

The Cloning, Isolation and Characterization of a Biologically Active Human Enzyme, Urokinase, in E. Coli

  • Chapter
  • First Online:
Eukaryotic Cell Cultures

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 172))

Abstract

We have cloned double-stranded cDNA copies of plasminogen activator messenger RNA isolated from human fetal kidney cells. Some of the clones express protein of discrete sizes ranging from 32,000 to 150,000 daltons. These products possess antigenic determinants related to human plasminogen activator from kidney cells, bind to an affinity column specific for serine protease and activate human plasminogen to dissolve fibrin clots.

This work was carried out while the author was at Abbott Laboratories, North Chicago, Illinois

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. J.R.B. Williams, Brit. J. Exptl. Pathol. 32:530–535, 1951.

    Google Scholar 

  2. M.B. Bernik and H.C. Kwaan, J. Lab. Clin. Med. 70:650–661, 1967.

    Google Scholar 

  3. R.O. Studer, G. Roncari and W. Lergier, In: Thrombosis and Urokinase, Paoletti, R. and Sherry S. (eds), Academic Press, p. 89-90, 1977.

    Google Scholar 

  4. A. Ulrich, J. Shine, J. Chirgwin, R. Pictet, E. Tirscher, W.J. Rutter and H.M. Goodman, Science 196:1313–1318, 1977.

    Article  Google Scholar 

  5. R.G. Deeley, J.I. Gordon, A. Burns, K.P. Mullinix, M. Bina-Stein and R.F. Goldberger, J. Biol. Chem. 252:8310–8319, 1977.

    Google Scholar 

  6. H.R. Pelham and R.J. Jackson, Eur. J. Biochem. 67:247–256, 1976.

    Article  Google Scholar 

  7. R.E. Rhodes, G.S. McKnight and R.T. Schmike, Biol. Chem. 248:2031–2039, 1973.

    Google Scholar 

  8. L. Clarke, R. Hitzeman and J. Carbon, In: Methods in Enzymology, Vol. 68, pp. 436–442, Academic Press, Inc., New York, 1979.

    Google Scholar 

  9. J. Unkeless, K. Dano, G.M. Kellerman and E. Keick, J. Biol. Chem., 249:4295–4305, 1974.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Genetics Division, Bethesda Research Laboratories, Inc., Gaithersburg, Maryland, 20760, USA

    P. P. Hung

Authors
  1. P. P. Hung
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Microbiology, University of Alabama in Birmingham, Birmingham, Alabama, USA

    Ronald T. Acton  & J. Daniel Lynn  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Plenum Press, New York

About this chapter

Cite this chapter

Hung, P.P. (1984). The Cloning, Isolation and Characterization of a Biologically Active Human Enzyme, Urokinase, in E. Coli. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9376-8_17

  • Published:

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-9378-2

  • Online ISBN: 978-1-4615-9376-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation