Abstract
The microcarrier cell culture technique offers a convenient way of manipulating relatively large amounts of anchorage-dependent cultured mammalian cells in a relatively small volume. Other advantages of this system are ease of direct observation, and of monitoring and control of other culture parameters. The production of human fibroblasts interferon on microcarrier culture on a scale of up to 44 litres (very roughly equivalent to 1100 roller bottles) using a conventional inducation with poly(I) poly(C), followed by superinduction by treatment with metalolic inhibitors, is described.
Large portions of the work described in this article were supported by Contract No. NO l-CM-07370 from the NCI.
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Abbreviations
- MEM:
-
Eagle’s minimal essential medium, (Flow Labs.)
- DME:
-
Dulbecco’s modified Eagle’s medium, (Flow Labs or Gibco)
- FBS:
-
Fetal bovine serum (Flow Labs.)
- DEAE:
-
Diethylaminoethyl
- poly(I) poly(C):
-
Polyriboinosinic acid — polyribocytidylic acid homopolymer duplex. (P-L Biochemicals)
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© 1984 Plenum Press, New York
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Edy, V.G. (1984). Interferon Production in Microcarrier Culture of Human Fibroblast Cells. In: Acton, R.T., Daniel Lynn, J. (eds) Eukaryotic Cell Cultures. Advances in Experimental Medicine and Biology, vol 172. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9376-8_10
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DOI: https://doi.org/10.1007/978-1-4615-9376-8_10
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