Abstract
Determination of the nucleic acid sequence of MHV genomic and subgenomic RNAs is clearly a way of predicting the primary structure of encoded polypeptides and at the same time identifying non-coding regions, which may be relevant to the regulation of the viral genes and to the replication of the viral RNA. A comparative analysis of the sequences of different MHV strains, or mutants with altered phenotypes, and of other coronaviruses will also be a method of identifying functionally important regions by their conserved sequences. Such analysis will also be required to relate changes in nucleotide sequence to changes, for example, in host range and pathogenicity.
Chapter PDF
Similar content being viewed by others
References
Siddell, S.G., Wege, H., Barthel, A. and ter Meulen, V. (1980). J. Virol. 33, 10–17.
Siddell, S., Wege, H., Barthel, A. and ter Meulen, V. (1981). J. Gen. Virol. 53, 145–155.
Stohlman, S.A. and Lai, M.M.C. (1979). J. Virol. 32, 672–675.
Siddell, S.G., Barthel, A. and ter Meulen, V. (1981). J. Gen. Virol. 52, 235–243.
Land, H., Grez, M., Hauser, H., Lindenmaier, W. and Schütz, G., (1983). In Methods in Enzymology, eds. Wu, R., Grossman, L. and Moldave, K. (Academic Press, New York), Vol. 100, in press.
Dagert, M. and Ehrlich, S.D. (1979). Gene 6, 23–28.
Holmes, D.S. and Quigley, M. (1981). Anal. Biochem. 114, 193–197.
Messing, J. and Vierira, J. (1982). Gene 19, 269–276.
Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA 74, 5463–5467.
Hu, N. and Messing, J. (1982). Gene 17, 271–277.
Paterson, B.M., Roberts, B.E. and Kuff, E.L. (1977). Proc. Nat. Acad. Sci. USA 74, 4370–4374.
Laemmli, U.K. (1970). Nature (London) 227, 680–685.
Garoff, H., Frischauf, A., Simons, K., Lehrach, H. and Delius, H. (1980). Proc. Natl. Acad. Sci. USA 77, 6376–6380.
Lai, M.M.C., Patton, C.D. and Stohlman, S.A. (1982). J. Virol. 41, 557–565.
Spaan, W.J.M., Rottier, P.J.M., Horzinek, M.C. and van der Zeijst, B.A.M. (1982). J. Virol. 42, 432–439.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Skinner, M., Siddell, S. (1984). Nucleotide Sequencing of Mouse Hepatitis Virus Strain JHM Messenger RNA 7. In: Rottier, P.J.M., van der Zeijst, B.A.M., Spaan, W.J.M., Horzinek, M.C. (eds) Molecular Biology and Pathogenesis of Coronaviruses. Advances in Experimental Medicine and Biology, vol 173. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9373-7_17
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9373-7_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9375-1
Online ISBN: 978-1-4615-9373-7
eBook Packages: Springer Book Archive