Skip to main content

Recent Advances in Protease Research using Synthetic Substrates

  • Chapter
Proteases

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 167))

Abstract

Until recently the use of synthetic peptides in protease research was rather limited; this was mainly due to the lack of suitable substrates and effective reaction principles (Pearse, 1972; Smith and van Frank, 1975; Lojda et al., 1979). However, especially during the last years substantial progress was achieved in this field; it is the aim of the present report to describe the most important advances in the use of synthetic peptide substrates.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Antonowicz, C., 1979, The role of enteropeptidase in the digestion of protein and its development in the human fetal small intestine, in: “Development of mammalian absorptive processes” (Ciba Foundation Symposion 70), Exerpta Medica, Amsterdam.

    Google Scholar 

  • Blenk, R., and Gossrau, R., 1981, Biochemische und quantitativ-histochemische Untersuchungen von Proteasen in der Gl. submandibular is von Ratten, Acta histochemica Suppl. 24:309.

    CAS  Google Scholar 

  • Carone, F. A., and Peterson, D.R., 1980, Hydrolysis and transport of small peptides by the proximal tubule, Am. J, Physiol. 238:151.

    Google Scholar 

  • Carone, F.A., Peterson, D.R., Oparil, S., and Pullman, T. N., 1980, Renal transport and catabolism of small peptides, in: “ Functional ultrastructure of the kidney,” A.B. Maunsbach, A. Steen Olsen and E.J. Christensen, eds., Academic Press, London, New York, Toronto, Sydney, San Francisco.

    Google Scholar 

  • Dolbeare, F.A., and Smith, R.E., 1977, Flow cytometric measurement of peptidases with use of 5-nitrosa-licylaldehyde and 4-methoxy-β-naphthylamine derivatives, Clin. Chem. 23:1485.

    PubMed  CAS  Google Scholar 

  • Dolbeare, F.A., and Vanderlaan, M., 1979, A fluorescent assay of proteinases in cultured mammalian cells, J. Histochem. Cytochem. 27: 1493.

    Article  PubMed  CAS  Google Scholar 

  • Emmanouel, D.S., Lindheimer, M.D., and Katz, A.J., 1980a, Pathogenesis of endocrine abnormalities in uremia, Endocr. Rev. 1: 28.

    Article  PubMed  CAS  Google Scholar 

  • Emmanouel, D.S., Lindheimer, M.D., and Katz, A.J., 1980b, Role of kidney in hormone metabolism and its implication in clinical medicine, Klin. Wochenschr. 58:1005.

    Article  PubMed  CAS  Google Scholar 

  • Gossrau, R., 1980, Conventional techniques for membranebound enzymes, in: “Trends in enzyme histochemistry and cytochemistry” (Ciba Foundation Symposion 73), Excerpta Medica, Amsterdam.

    Google Scholar 

  • Gossrau, R., 1981, Investigation of proteinases in the digestive tract using 4-methoxy-2-naphthylamine (MNA) substrates, J. Histochem. Cytochem. 29:464.

    Article  PubMed  CAS  Google Scholar 

  • Gossrau, R., 1982a, Localization and regulation of proteolytic enzymes in salivary glands, submitted.

    Google Scholar 

  • Gossrau, R., 1982b, Peptide and protein handling by proteases in the kidney of laboratory rodents, Acta histochem. Suppl., in press.

    Google Scholar 

  • Gossrau, R., and Lojda, Z., 1980, Study on dipeptidylaminopeptidase II (DAP II), Histochemistry 70:53.

    Article  PubMed  CAS  Google Scholar 

  • Gutschmidt, S., and Gossrau, R., 1981, A quantitative histochemical study of dipeptidylpeptidase (DPP IV), Histochemistry 73:285.

    Article  PubMed  CAS  Google Scholar 

  • Hartmann, K., and Gossrau, R., 1982, Zur Eignung von Aminomethylcoumarin-und Naphthylaminsubstraten für mikrochemische Peptidasenmessungen, Acta histochemica Suppl., in press.

    Google Scholar 

  • Hesford, F., Hadorn, B., Blaser, K., and Schneider, C.H., 1976, A new substrate for enteropeptidase, FEBS (Fed. Eur. Biochem. Soc.) Lett. 71: 279.

    Article  CAS  Google Scholar 

  • Hess, R., 1965, Arylamidase activity related to angiotensinase, Biochim. Biophys. Acta 99: 316.

    Article  PubMed  CAS  Google Scholar 

  • Huseby, R.M., and Smith, R.E., 1980, Synthetic oligopeptide substrates — their diagnostic application in blood coagulation, fibrinolysis, and other pathological states, Seminars in Thrombosis and Hemostasis 6:173.

    Article  CAS  Google Scholar 

  • Kanaoka, Y., Takahashi, T., and Nakayama, H., 1977, A new fluorogenic substrate for aminopeptidase, Chem. Pharm. Bull. 25:362.

    Article  PubMed  CAS  Google Scholar 

  • Kugler, P., 1982, Aminopeptidase A is angiotensinase A. I. Quantitative histochemical studies in the kidney glomerulus, Histochemistry 74:229.

    Article  PubMed  CAS  Google Scholar 

  • Lojda, Z., 1981, Proteinases in pathology. Usefulness of histochemical methods, J. Histochem. Cytochem. 29:481.

    Article  PubMed  CAS  Google Scholar 

  • Lojda, Z., and Gossrau, R., 1980, Study on aminopeptidase A, Histochemistry 67: 267.

    Article  PubMed  CAS  Google Scholar 

  • Lojda, Z., and Gossrau, R., 1982, Histochemical demonstration of enteropeptidase. New method with a synthetic substrate and its comparison with the trypsinogen procedure, Histochemistry, in press.

    Google Scholar 

  • Lojda, Z., Gossrau, R., and Schiebler, T.H., 1979, Enzyme histochemistry. A laboratory manual, Springer, Berlin, Heidelberg, New York.

    Book  Google Scholar 

  • Lojda, Z., and Kuhlich, J., 1981, The usefulness of analytical electrofocusing in a thin-layer polyacryl-amide gel (PAG) in the histochemistry of enzymes cleaving peptide bonds, Histochemistry 73:311.

    Article  PubMed  CAS  Google Scholar 

  • Maunsbach, A.B., Steen Olsen, T., and Christensen, E.J., eds., 1980, Functional ultrastructure of the kidney, Academic Press, London, New York, Toronto, Sidney, San Francisco.

    Google Scholar 

  • Nagatsu, I.T., Gillespie, I.L., George, J.M., Folk, J.E., and Glenner, G.G., 1965, Serum aminopeptidase “angiotensinase” and hypertension. — II. Amino acid β-naphthylamide hydrolysis by normal and hypertensive serum, Biochem. Pharmacol., 14:853.

    Article  PubMed  CAS  Google Scholar 

  • Nagatsu, I.T., Yamamoto, T., Glenner, G.G., and Mehl, J.W., 1970, Purification of aminopeptidase A in human serum and degradation of angiotensin II by the purified enzyme, Biochem. Biophys. Acta 198:255.

    Article  PubMed  CAS  Google Scholar 

  • Pearse, A.G.E., 1972, “Histochemistry. Theoretical and applied,” Churchill Livingstone, Edinburgh.

    Google Scholar 

  • Schröder, J., and Gossrau, R., 1982, Elektronenmikroskopische Lokalisation von Proteasen mit direkten Azofarbstoffmethoden, Acta histochemica Suppl., 25:147.

    Google Scholar 

  • Sinha, P., and Gossrau, R., 1982, Histochemical studies with MNA substrates using isoelectric focusing, J. Histochem. Cytochem. 30:575.

    Google Scholar 

  • Smith, R.E., Bissell, E.R., Mitchell, A.R., and Pearson, K.W., 1980, Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin, Thromb. Res. 17:393.

    Article  PubMed  CAS  Google Scholar 

  • Smith, R.E., and van Frank, R.M., 1975, The use of amino acid derivatives of 4-methoxy-β-naphthylamine for the assay and subcellular localization of tissue proteinases, in: “Lysosoms in biology and pathology,” Vol. 4, J.T. Dingle and R.J. Dean, eds., North Holland Publishing Company, Amsterdam.

    Google Scholar 

  • Smith, R.E., and Grabske, R.J., 1982, Isoenzymes of glandular kallikrein revealed by isoelectric focusing and fluorescence staining with an oligopeptide substrate inpregnated into cellulose, J. Histochem. Cytochem. 30: 575.

    Google Scholar 

  • Stauber, W.T., and Ong, S.H., 1981, Fluorescence demonstration of cathepsin B in skeletal, cardiac, and vascular smooth muscle, J. Histochem. Cytochem. 29:866.

    Article  PubMed  CAS  Google Scholar 

  • Zimmerman, M., Yurewicz, E., and Patel, G., 1976, A new fluorogenic substrate for chymotrypsin, Anal. Biochem. 70:258.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Plenum Press, New York

About this chapter

Cite this chapter

Gossrau, R., Lojda, Z., Smith, R.E., Sinha, P. (1984). Recent Advances in Protease Research using Synthetic Substrates. In: Hörl, W.H., Heidland, A. (eds) Proteases. Advances in Experimental Medicine and Biology, vol 167. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9355-3_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9355-3_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9357-7

  • Online ISBN: 978-1-4615-9355-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics