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SteroidΔ1-Dehydrogenase Isoenzymes in Corynebacterium Species Chol 73 T 191

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Enzyme Engineering

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Abstract

Corynebacterium spec, mutant Chol 73 T 191 degrades cholesterol nearly quantitatively into 20-carboxy-pregna-1,4-diene-3-one (BNC) in the absence of inhibitors (1). From the presence of small amounts of 20-carboxy-pregn-4-ene-3-one (Δ4-BNC), androsta-1,4-diene-3,17-dione (AD) and androst-4-ene-3, 17-dione (ADD), the presence of a steroid Δ1-dehydrogenase (E.C. 1.3.99.4) can be inferred. This enzyme has recently been isolated and purified 160 fold from Nocardia opaca (2). In Mycobacterium in direct evidence for the presence of an isoenzyme system has been presented (3). We now report direct proof for steroid Δ1-dehydrogenase isoenzymes inCorynebacterium spec. Chol 73 T 191, based on the RB position of the specifically stained steroid Δ1-dehydrogenase band after PAA-electrophoresis with bromophenol blue having an RB value of 1.00. With AD and cholesterol as inducers, the RB values were 0.09 and 0.71, respectively.

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References

  1. Hill, F., Preuss, W., Schindler, J. & Schmid, R. D., Eur. Patent Appl. 4913, 1979.

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  2. LESTROVAJA, N. N., DANHARDT, S., & HORHOLD, C. Z. Allg. Mikrobiol. 18: 189 (1978).

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Author information

Authors and Affiliations

  1. Departments of Biotechnology, Fat Research HENKEL KGaA, Duesseldorf, Fed. Rep. Germany

    W.-R. Mueller, W. Preuss & R. D. Schmid

Authors
  1. W.-R. Mueller
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  2. W. Preuss
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  3. R. D. Schmid
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Editor information

Editors and Affiliations

  1. Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company, Osaka, Japan

    Ichiro Chibata

  2. Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan

    Saburo Fukui

  3. Department of Pharmacology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Lemuel B. Wingard Jr.

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© 1982 Plenum Press, New York

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Mueller, WR., Preuss, W., Schmid, R.D. (1982). SteroidΔ1-Dehydrogenase Isoenzymes in Corynebacterium Species Chol 73 T 191. In: Chibata, I., Fukui, S., Wingard, L.B. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9290-7_16

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  • DOI: https://doi.org/10.1007/978-1-4615-9290-7_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9292-1

  • Online ISBN: 978-1-4615-9290-7

  • eBook Packages: Springer Book Archive

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