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Speed-Dependent Sedimentation-Velocity of Human Cervical Mucins in the Analytical Ultracentrifuge

  • John Sheehan
  • Ingemar Carlstedt
Chapter
  • 142 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 144)

Abstract

Highly purified human cervical mucins were prepared by isopycnic density-gradient centrifugation (Carlstedt et al., 1981). The macro-molecules elute in the void volume of a Sepharose CL 2B column and five sepgrate preparations gave molecular weights between 4.8 and 6.5 × 106 by light-scattering in 6M guanidine hydrochloride (GuHCl). To study the size heterogeneity and polydispersity, sedimentation-velocity experiments were carried out in the analytical ultracentrifuge. In the course of this work new observations were made on the sedimentation behaviour of large mucin molecules.

Keywords

Void Volume Guanidine Hydrochloride Edge Angle Knife Edge Analytical Ultracentrifuge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aten, J.B.T. and Cohen, J.A.,1965, J. Mol. Biol.,12: 537.PubMedCrossRefGoogle Scholar
  2. Carlstedt, I., Sheehan, J. and Wingerup, L., 1981, Proceedings of the Second Symposium on Mucus in Health and Disease.Google Scholar
  3. Schumaker, V.N. and Zimm, B.H.,1973, Biopolymers, 12: 869, 877.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • John Sheehan
    • 1
  • Ingemar Carlstedt
    • 1
  1. 1.Department of Physiological Chemistry 2University of LundLund 7Sweden

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