Speed-Dependent Sedimentation-Velocity of Human Cervical Mucins in the Analytical Ultracentrifuge
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Highly purified human cervical mucins were prepared by isopycnic density-gradient centrifugation (Carlstedt et al., 1981). The macro-molecules elute in the void volume of a Sepharose CL 2B column and five sepgrate preparations gave molecular weights between 4.8 and 6.5 × 106 by light-scattering in 6M guanidine hydrochloride (GuHCl). To study the size heterogeneity and polydispersity, sedimentation-velocity experiments were carried out in the analytical ultracentrifuge. In the course of this work new observations were made on the sedimentation behaviour of large mucin molecules.
KeywordsVoid Volume Guanidine Hydrochloride Edge Angle Knife Edge Analytical Ultracentrifuge
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- Carlstedt, I., Sheehan, J. and Wingerup, L., 1981, Proceedings of the Second Symposium on Mucus in Health and Disease.Google Scholar