The Use of Radioimmunoassay as a Probe of the Antigenic Determinants and Structure of Human Small Intestinal Mucin
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Native undegraded mucins from a variety of epithelial tissues in different animal species have been shown to be remarkably similar in their overall chemical composition, containing the same complement of sugars and spectrum of amino acids. Many have a high molecular weight, similar rheological properties and are sensitive to thiol reduction. It was considered possible, however, that subtle but significant differences in the structure and chemical features of mucins might be detected by immunological techniques. To this end, a solid phase radioimmunoassay (RIA) for human small intestinal (HSI) mucin has been developed. The RIA was used to compare the antigenic activity of various mucins and haptens, and to study the effects of thiol reduction upon HSI mucin.
KeywordsAntigenic Determinant Human Small Intestinal Mucus Glycoprotein Solid Phase Radioimmunoassay Mucin Protein
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