Abstract
Native undegraded mucins from a variety of epithelial tissues in different animal species have been shown to be remarkably similar in their overall chemical composition, containing the same complement of sugars and spectrum of amino acids. Many have a high molecular weight, similar rheological properties and are sensitive to thiol reduction. It was considered possible, however, that subtle but significant differences in the structure and chemical features of mucins might be detected by immunological techniques. To this end, a solid phase radioimmunoassay (RIA) for human small intestinal (HSI) mucin has been developed. The RIA was used to compare the antigenic activity of various mucins and haptens, and to study the effects of thiol reduction upon HSI mucin.
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© 1982 Plenum Press, New York
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Mantle, M., Wesley, A.W., Forstner, J.F. (1982). The Use of Radioimmunoassay as a Probe of the Antigenic Determinants and Structure of Human Small Intestinal Mucin. In: Chantler, E.N., Elder, J.B., Elstein, M. (eds) Mucus in Health and Disease—II. Advances in Experimental Medicine and Biology, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9254-9_4
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DOI: https://doi.org/10.1007/978-1-4615-9254-9_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-9256-3
Online ISBN: 978-1-4615-9254-9
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