Studies of the Protective Properties of Gastric Mucus
- 145 Downloads
It is often assumed that the mucus layer protects gastric mucosa from damage by intraluminal acid and pepsin. However the mechanism by which it might achieve this is uncertain. We examined therefore the ability of gastric mucus to resist the diffusion across it of hydrogen and other small ions. Pig gastric mucus was gently scraped off freshly slaughtered pigs stomachs. The mucus was gently washed with isotonic saline to remove adherent food particles and small samples were layered between gauze supports in a perspex flux chamber. The layer of mucus, approximately 2.5 mm thick, separated equimolar solutions of HC1, on one side, and NaCl on the other (154 mM). The solutions were bubbled with 100% oxygen to ensure adequate mixing and were maintained at 20°C. The pH on the saline side was monitored with a pH probe placed within the solution and samples were taken simultaneously from both sides for estimation of sodium concentrations.