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Differences in Mucus Glycoproteins of Small Intestine from Subjects With and Without Cystic Fibrosis

  • A. W. Wesley
  • A. R. Qureshi
  • G. G. Forstner
  • J. F. Forstner
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 144)

Abstract

Studies on the composition of mucus from subjects with Cystic Fibrosis (CF) have previously shown increased fucose and decreased sialic acid (Dische et al, 1959; Clamp and Gough, 1979). To expand these studies we isolated human intestinal goblet cell mucus as previously described (I. Jabbal et al, 1976) with further purification by nuclease enzyme digestion and Sepharose 2B column chromatography. Mucins from 4 subjects with CF aged 9‒23 years and 6 non CF controls aged 1 day to 90 years were compared with respect to amino acid, carbohydrate content and CsCl density gradient ultra-centrifugation.

Keywords

Cystic Fibrosis Sialic Acid Cystic Fibrosis Patient Total Carbohydrate Buoyant Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Clamp, J.R., and Gough, M., 1979, Study of the oligosaccharide units from mucus glycoproteins of meconium from normal infants and from cases of cystic fibrosis with meconium ileus, Clin. Sci., 57: 445.PubMedGoogle Scholar
  2. Dische, Z., di Sant’Agnese, P., Pallavichini, C, Youlos, J., 1959, Compositions of mucoprotein fractions from duodenal fluid of patients with cystic fibrosis of the pancreas and from controls, Pediatrics, 24: 74.PubMedGoogle Scholar
  3. Jabbal, I., Kells, D.I.C., Forstner, G., Forstner, J., 1976, Human intestinal goblet cell mucin, Can. J. Biochem., 54: 707.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • A. W. Wesley
    • 1
  • A. R. Qureshi
    • 1
  • G. G. Forstner
    • 1
  • J. F. Forstner
    • 1
  1. 1.The Hospital for Sick ChildrenTorontoCanada

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