Differences in Mucus Glycoproteins of Small Intestine from Subjects With and Without Cystic Fibrosis
- 145 Downloads
Studies on the composition of mucus from subjects with Cystic Fibrosis (CF) have previously shown increased fucose and decreased sialic acid (Dische et al, 1959; Clamp and Gough, 1979). To expand these studies we isolated human intestinal goblet cell mucus as previously described (I. Jabbal et al, 1976) with further purification by nuclease enzyme digestion and Sepharose 2B column chromatography. Mucins from 4 subjects with CF aged 9‒23 years and 6 non CF controls aged 1 day to 90 years were compared with respect to amino acid, carbohydrate content and CsCl density gradient ultra-centrifugation.