Abstract
Studies on the composition of mucus from subjects with Cystic Fibrosis (CF) have previously shown increased fucose and decreased sialic acid (Dische et al, 1959; Clamp and Gough, 1979). To expand these studies we isolated human intestinal goblet cell mucus as previously described (I. Jabbal et al, 1976) with further purification by nuclease enzyme digestion and Sepharose 2B column chromatography. Mucins from 4 subjects with CF aged 9‒23 years and 6 non CF controls aged 1 day to 90 years were compared with respect to amino acid, carbohydrate content and CsCl density gradient ultra-centrifugation.
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References
Clamp, J.R., and Gough, M., 1979, Study of the oligosaccharide units from mucus glycoproteins of meconium from normal infants and from cases of cystic fibrosis with meconium ileus, Clin. Sci., 57: 445.
Dische, Z., di Sant’Agnese, P., Pallavichini, C, Youlos, J., 1959, Compositions of mucoprotein fractions from duodenal fluid of patients with cystic fibrosis of the pancreas and from controls, Pediatrics, 24: 74.
Jabbal, I., Kells, D.I.C., Forstner, G., Forstner, J., 1976, Human intestinal goblet cell mucin, Can. J. Biochem., 54: 707.
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© 1982 Plenum Press, New York
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Wesley, A.W., Qureshi, A.R., Forstner, G.G., Forstner, J.F. (1982). Differences in Mucus Glycoproteins of Small Intestine from Subjects With and Without Cystic Fibrosis. In: Chantler, E.N., Elder, J.B., Elstein, M. (eds) Mucus in Health and Disease—II. Advances in Experimental Medicine and Biology, vol 144. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9254-9_19
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DOI: https://doi.org/10.1007/978-1-4615-9254-9_19
Publisher Name: Springer, Boston, MA
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