The Use of High Resolution Carbon-13 NMR in the Study of Mucus
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The glycoproteins of mucus secretions are large polymers with molecular weights of several million daltons. The molecules are believed to have a peptide backbone with oligosaccharide chains which conform to a bottlebrush structure. Physical investigations on mucus glycoproteins have been hampered by the presence of co-secreted proteins. The presence of these proteins means that purification of the glycoprotein is generally needed. Several methods of purification have been used which have yielded preparations which can be studied by chemical and physical methods e.g. density gradient centrifugation (1) and gel exclusion chromatography (2). The work described here utilises carbon-13 NMR on native mucus and glycoprotein obtained from both centrifugation and gel chromatography.
KeywordsSialic Acid Mucus Secretion Methyl Signal Oligosaccharide Chain Extraneous Material
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