Advertisement

The Use of High Resolution Carbon-13 NMR in the Study of Mucus

  • K. Barrett-Bee
  • G. Bedford
  • P. Loftus
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 144)

Abstract

The glycoproteins of mucus secretions are large polymers with molecular weights of several million daltons. The molecules are believed to have a peptide backbone with oligosaccharide chains which conform to a bottlebrush structure. Physical investigations on mucus glycoproteins have been hampered by the presence of co-secreted proteins. The presence of these proteins means that purification of the glycoprotein is generally needed. Several methods of purification have been used which have yielded preparations which can be studied by chemical and physical methods e.g. density gradient centrifugation (1) and gel exclusion chromatography (2). The work described here utilises carbon-13 NMR on native mucus and glycoprotein obtained from both centrifugation and gel chromatography.

Keywords

Sialic Acid Mucus Secretion Methyl Signal Oligosaccharide Chain Extraneous Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. (1).
    Creeth. Characterization of secretory glycoproteins by ultracentrifuge methods in “Glycoconjugates” 94. ed. Schauer, George Thieme Stuttgart (1979).Google Scholar
  2. (2).
    Marriott, Barrett-Bee, Brown. A comparative evaluation of mucus from different sources. Ibid 528 (1979).Google Scholar
  3. (3).
    Wardell, Chakrin, Payne. Am. Rev. Resp. Dis. 101, 741 (1970).Google Scholar
  4. (4).
    Voelter, Bilik, Breitmaier. Collect. Czech. Chem. Comm. 38. 2054 (1973).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • K. Barrett-Bee
    • 1
  • G. Bedford
    • 1
  • P. Loftus
    • 1
  1. 1.ICI Pharmaceuticals DivisionAlderley Park MacclesfieldCheshireUK

Personalised recommendations