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Biosynthesis of Mucus Glycoproteins

  • Harry Schachter
  • David Williams
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 144)

Abstract

Mucus in higher organisms is usually defined as the viscous fluid lining the epithelium of the gastro-intestinal, respiratory and genito-urinary tracts. Mucus is a complex mixture containing large glycoproteins (mucins), water, electrolytes, sloughed epithelial cells, enzymes and various other materials, including bacteria and bacterial products depending on the source and location of the mucus. This discussion will be limited to a survey of the biosynthesis of the carbohydrate portion of mucins, the major components of mucus. Mucins are large glycoproteins (molecular weight is usually over 1 × 106> ) containing from 50 to 80% or more by weight of carbohydrate. As will be discussed in more detail below, the carbohydrate is linked to the polypeptide by an O-glycosidic bond between GalNAc and Ser or Thr. Mucins are polydisperse with respect to molecular size and oligosaccharide sequences and chain lengths. Thus structural information on mucin oligosaccharides must be obtained by cleaving the Ser(Thr)-GalNAc bond, usually by alkali-catalyzed β-elimination, and purifying individual oligosaccharides. By analogy, our discussion of biosynthesis will deal with the assembly of a single oligosaccharide at a time.

Keywords

Sialic Acid Blood Group Submaxillary Gland Sendai Virus Gastric Mucin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Harry Schachter
    • 1
  • David Williams
    • 1
  1. 1.Research Institute, Hospital for Sick Children, and Department of BiochemistryUniversity of TorontoTorontoCanada

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