Abstract
Direct measurements of ion concentration changes in viv. became possible after the development of ion-selective microelectrodes (ISM) with a liquid ion-exchanger located in the shank of a glass micropipette tip (Walker, 1971). For potassium ISM, an organic resin Corning code 477317 was used as the ion-exchanger. This ion-exchanger employs 1-2-dimethyl-3-nitrobenzen for dissolving 2g/100 ml potassium tetra-kis (p-chlorophenyl)borate (Baum and Wise, 1971). The relative selectivities of Corning ion-exchanger for some cations were first shown by Wise et al.(1970). The selectivities of the K+-electrode for some interferring ions with respect to the principal ion were also determined by Moody and Thomas (1971), Walker (1971), Khuri et al. (1972) and others. Electrode potentials were measured in mixtures of constant ionic strength and appropriate selectivity constants were calculated — KK, Ca = 0.002, KK,H = 0.025, KK, Ca = 0.02, which are valid for ionic strength 0.1 mol.1−1 (Walker, 1971).
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© 1981 Plenum Press, New York
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Kříž, N., Syková, E. (1981). Sensitivity of K+-Selective Microelectrodes to pH and Some Biologically Active Substances. In: Syková, E., Hník, P., Vyklický, L. (eds) Ion-Selective Microelectrodes and Their Use in Excitable Tissues. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9224-2_3
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DOI: https://doi.org/10.1007/978-1-4615-9224-2_3
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