Summary
We describe the application to flow systems of one-dimensional image analysis by slit-scanning and dual laser excitation for in creased spectral resolution. The former allows morphological identification of large metaphase chromosomes, increased resolution in flow karyotypes, and detection of chromosome aberrations. The dual laser system and appropriate detection optics facilitates the use of resonance energy transfer to probe the topological properties of cell surface components. As an example, we discuss the receptors for the lectin concanavalin A.
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© 1982 Plenum Press, New York
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Jovin, T.M., Arndt-Jovin, D.J. (1982). Flow Sorting on the Basis of Morphology and Topology. In: Hélène, C., Charlier, M., Montenay-Garestier, T., Laustriat, G. (eds) Trends in Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9203-7_6
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DOI: https://doi.org/10.1007/978-1-4615-9203-7_6
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