Abstract
In spite of the fact that thymus-derived lymphocytes (T cells) bind antigens specifically (1), interaction between the surface receptor and antigen on these cells has not been directly visualized as it has been for bone marrow-derived lymphocytes (B cells) (2–4). Several investigators have recently established that avian antibodies specific for mammalian Ig bind to T cells, as well as to B cells, and that this binding is demonstrable by immunofluorescence (5–8). If the T cell surface components visualized by avian anti-mammalian Ig are involved in primary immune recognition, these components should be coincidentally distributed with antigen on the surface of ABL from thymus.
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Abbreviations
- ABL:
-
antigen-binding lymphocyte(s)
- CAM:
-
(Fab′)2 chicken anti-mouse (Fab′)2 fragments
- DMSO:
-
dimethyl sulfoxide
- FCS:
-
fetal calf serum
- FITC:
-
fluorescein isothiocyanate
- HBSS:
-
Hanks balanced salt solution
- HSF:
-
horse spleen ferritin
- Ig:
-
immunoglobulin(s)
- KLH:
-
keyhole limpet hemocyanin
- MHC:
-
major histocompatibility complex
- NCG:
-
normal chicken globulin
- PBS:
-
phosphate buffered saline
- RACG:
-
rabbit anti-chicken globulin
- RAMG:
-
rabbit anti-mouse globulin
- TRITC:
-
tetramethyl-rhodamine isothiocyanate
- NMγG:
-
normal mouse gamma globulin
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© 1979 Plenum Press, New York
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DeLuca, D., Warr, G.W., Marchalonis, J.J. (1979). Codistribution of Antigen and Fab Determinants on Thymic Antigen-Binding Cells. In: Müller-Ruchholtz, W., Müller-Hermelink, H.K. (eds) Function and Structure of the Immune System. Advances in Experimental Medicine and Biology, vol 114. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9101-6_24
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DOI: https://doi.org/10.1007/978-1-4615-9101-6_24
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