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Methods of Cell Separation pp 67–91Cite as

Electrophoretic Light Scattering and Its Application to the Study of Cells

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Part of the book series: Biological Separations ((BIOSEP))

Abstract

During recent years researchers have shown that the physical properties of cells can be related to their biological function and that different cell types can be identified and separated by using appropriate physical parameters as distinguishing factors. Such properties as cell size or volume (Miller and Phillips, 1969), density (Shortman, 1968), cell surface markers (Raff, 1971; Zeiller et al., 1974), and surface charge (Dumont, 1974) have been successfully used to distinguish functionally different cells. In this report, the principles of a relatively new electrophoresis technique, referred to as electrophoretic light scattering (ELS) (Ware and Flygare, 1971; Ware, 1974; Flygare et al., 1976) are described. With this technique, the electrophoretic mobility distribution (refer to section II.A) can be measured for a large number of cells, in a small sample volume (0.1 ml), in a fraction of the time required by classical methods. A cell’s electrophoretic mobility reflects its surface charge and thus its surface composition. For example, functional changes in a cell are often correlated with changes in the cell’s electrophoretic mobility, indicating an alteration in the surface charge and possibly the composition of the membrane (Smith et al., 1976).

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Authors and Affiliations

  1. Xerox Research Centre of Canada, Mississauga, Ontario, L5L 1J9, Canada

    Jack Y. Josefowicz

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  1. Jack Y. Josefowicz
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Editors and Affiliations

  1. Massachusetts Institute of Technology, USA

    Nicholas Catsimpoolas

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© 1979 Plenum Press, New York

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Josefowicz, J.Y. (1979). Electrophoretic Light Scattering and Its Application to the Study of Cells. In: Catsimpoolas, N. (eds) Methods of Cell Separation. Biological Separations. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9095-8_2

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  • DOI: https://doi.org/10.1007/978-1-4615-9095-8_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9097-2

  • Online ISBN: 978-1-4615-9095-8

  • eBook Packages: Springer Book Archive

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