Abstract
The loss of biological activity upon heating or agitation of human interferons is markedly altered by changing their aqueous environment. Low pH significantly stabilizes liquid fibroblast interferon at 68°C and 37°C whereas chaotropic salts stabilize at 68°C but not at 37°C; this anomalous result may be due to reactivation of biological activity at the higher temperature. The concentration of extraneous proteins influences the apparent thermal stability at any temperature and pH; thus, interferon was not stable even at low pH at protein concentrations less than 5 μg/ml. Solutions of partially purtially purified fibroblast interferon can be inactivated by mechanical stress; the addition of proteins or nonionic detergents prevents such inactivation. Freeze-dried preparations show the greatest thermal stability. The use of high-temperature, accelerated storage tests makes it possible to predict the shelf-life of freeze-dried interferon.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fantes, K. H. 1973. Purification and physico-chemical properties of interferons. Pgs. 171–200 in N. B. Finter, ed. Interferons and Interferon Inducers. North-Holland Publishing Co., New York.
Ng, M. H., and J. Vilcek. 1972. Interferons: physico-chemical properties and control of cellular synthesis. Advances in Protein Chemistry. 26: 173–239.
Cesario, T. C., P. J. Schryer and J. G. Tilles. 1977. Relationship between the physiochemical nature of human interferon, the cell induced and the inducing agent. Antimicrobial Agents and Chemotherapy 11: 291–298.
Mogensen, K. E. and K. Cantell. 1974. Human leukocyte interferon: a role for disulphide bonds. J. Gen. Virol. 22: 95–103.
Stewart, W. E. II, P. De Somer, V. G. Edy, et al. 1975. Distinct molecular species of human interferons: requirements for stabilization and reactivation of human leukocyte and fibroblast interferons. J. Gen. Virol. 26: 327–331.
Valle, M. J., G. W. Jordan, S. Haahr, et al. 1975. Characteristics of immune interferon produced by human lymphocyte cultures compared to other human interferons. J. Immunology 115: 230–232.
Edy, V. G., A. Billiau, M. Joniau et al. 1974. Stabilization of mouse and human interferons by acid pH against inactivation due to shaking and guanidine hydrochloride. Proc. Soc. Exp. Biol. Med. 146: 249–253.
Cartwright, T., O. Senussi and M.D. Grady. 1977. The mechanism of the inactivation of human fibroblast interferon by mechanical stress. J. Gen. Virol, in press.
Mogensen, K. E. and K. Cantell. 1973. Stability of human leukocyte interferon towards heat. Acta Path. Microbiol. Scand. Section B. 81: 382–383.
Knight, E., Jr. 1976. Interferon: purification and initial characterization from human diploid cells. Proc. Nat. Acad. Sci. USA 73: 520–523.
Berman, B. and J. Vilcek. 1974. Cellular binding characteristics of human interferon. Virology 57: 378–386.
Anfinsen, C. B., S. Bose, L. Corley, and D. Gurari-Rotman. 1974. Partial purification of human interferon by affinity chromatography. Proc. Nat. Acad. Sci. USA 71: 3139–3142.
Jameson, P., M. A. Dixon, and S. E. Grossberg. 1977. A sensitive interferon assay for many species of cells: encephalo- myocarditis virus hemagglutinin yield reduction. Proc. Soc. Exp. Biol. Med. 55: 173–178.
Greiff, D. and C. Grieff. 1972. Linear nonisothermal single- step, stability studies of dried preparations of influenza virus. Cryobiology 9: 34–37.
Jariwalla, R. J., S. E. Grossberg and J.J. Sedmak. 1977. Effect of chaotropic salts and protein dénaturants on the thermal stability of mouse fibroblast interferon. J. Gen. Virol. 35: 45–52.
De Somer, P., M. Joniau, V.G. Edy et al. 1974. Mass production of human interferon in diploid cells. Pgs. 39–46 in C. Waymouth, ed. The Production and Use of Interferon for the Treatment and Prevention of Human Virus Infections. The Tissue Culture Association, Rockville, MD.
Havell, E. A. and J. Vilcek. 1974. Mass production and some characteristics of human interferon from diploid cells. Pg. 47 in C. Waymouth, ed. The Production and Use of Interferon for the Treatment and Prevention of Human Virus Infections. The Tissue Culture Association, Rockville, MD.
Cartwright, T., O. Senussi and M. D. Grady. 1977. Reagents which inhibit disulphide bond formation stabilise human fibroblast interferon. J. Gen. Virol, in press.
Vilcek, J., E. A. Havell, and S. Yamazaki. 1977. Antigenic physico-chemical and biological characterization of human interferons. Annals of the New York Academy of Sciences 284: 703–710.
Tanford, C. 1978. Protein denaturation. Parts A and B. Adv. Prot. Chem. 23: 122–275.
Sedmak, J. J. and S. E. Grossberg. 1977. Stabilization of Interferons. Texas Reports on Biology and Medicine. In press.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Plenum Press, New York
About this chapter
Cite this chapter
Sedmak, J.J., Jameson, P., Grossberg, S.E. (1978). Thermal and Vortical Stability of Purified Human Fibroblast Interferon. In: Stinebring, W.R., Chapple, P.J. (eds) Human Interferon. Advances in Experimental Medicine and Biology, vol 110. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-9080-4_11
Download citation
DOI: https://doi.org/10.1007/978-1-4615-9080-4_11
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4615-9082-8
Online ISBN: 978-1-4615-9080-4
eBook Packages: Springer Book Archive