Mode of Action of Pancreatic Colipase

Abstract

Pancreatic lipase is inactivated by bile salts. This is a general detergent effect and depends on a displacement of lipase from the substrate interface resulting in a physical separation of lipase from the substrate (1,2). Colipase is a polypeptide of pancreatic origin that stays at the substrate interface even in the presence of bile salt and makes possible the interaction of lipase with the substrate in the presence of bile salt (3). To understand these complex interactions it is important to know the mechanisms of the separate part-reactions and I will deal with recent results of studies on the interaction between lipase and substrate, colipase and substrate and lipase and colipase. Finally the results of some chemical modification of colipase on the binding to lipase will be discussed.