Abstract
The isolation of lysosomes from brain, more so than from such other tissues as liver, encounters difficulties (1). Despite these difficulties lysosome enriched fractions can be prepared from brain homogenates by a combination of differential pelleting and density gradient centrifugation. Several reports dealing with the isolation of subcellular particles in brain tissue of different mammalia have been published (1,2).
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© 1978 Plenum Press, New York
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Overdijk, B., Hooghwinkel, G.J.M., Lisman, J.J.W. (1978). Isolation of Lysosomes from Bovine Brain Tissue a New Zonal Centrifugation Technique. In: Gatt, S., Freysz, L., Mandel, P. (eds) Enzymes of Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 101. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9071-2_56
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DOI: https://doi.org/10.1007/978-1-4615-9071-2_56
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