Isolation of Lysosomes from Bovine Brain Tissue a New Zonal Centrifugation Technique

  • B. Overdijk
  • G. J. M. Hooghwinkel
  • J. J. W. Lisman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 101)


The isolation of lysosomes from brain, more so than from such other tissues as liver, encounters difficulties (1). Despite these difficulties lysosome enriched fractions can be prepared from brain homogenates by a combination of differential pelleting and density gradient centrifugation. Several reports dealing with the isolation of subcellular particles in brain tissue of different mammalia have been published (1,2).


Sedimentation Rate Lysosomal Enzyme Sucrose Gradient Purification Factor Colloidal Iron 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • B. Overdijk
    • 1
  • G. J. M. Hooghwinkel
    • 1
  • J. J. W. Lisman
    • 1
  1. 1.Lab. of Chemical PhysiologyVrije UniversiteitAmsterdamThe Netherlands

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