Inhibitors of β-Glucosidases of Animal Tissues

  • Norman S. Radin
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 101)


Our first effort in the field of glucocerebroside manip­ulation stemmed directly from an observation by one of this meeting’s sponsors, Dr. Shimon Gatt. Dr. Gatt had purified glucocerebrosidase from brain to a certain degree and, in studying its properties, found that sphingosine produced good inhibition (1). It seemed likely that we could increase the strength and specificity of the effect by adding a glucosyl moiety to the sphingosine. John Erickson and I prepared glu­cosyl sphingosine from Gaucher spleen cerebroside and found that it was indeed a stronger inhibitor (2). When the com­pounds were tested with rat spleen cerebrosidase at the 0.3 mM level, sphingosine produced 59% inhibition and the glucoside produced 75% inhibition. Galactosyl sphingosine, the β-galac­toside made from brain cerebroside, had no effect at all, showing that the sugar residue was very important.


Glucose Moiety Glucosidase Activity Glucosidase Inhibitor Gauche Patient Glucosyl Moiety 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Norman S. Radin
    • 1
  1. 1.Mental Health Research Institute & Department of Biological ChemistryThe University of MichiganAnn ArborUSA

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