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Effect of Detergents on Ceramide-3 Hydrolysis by α-Galactosidase A

  • André W. Schram
  • Mae Wan Ho
  • Sylvia Cordus
  • Marieke Samson
  • Mic N. Hamers
  • Jan Brown
  • Don Robinson
  • Joseph M. Tager
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 101)

Abstract

The lysosmal hydrolase α-galactosidase A is responsible for the hydrolysis of several α 1→4 galactosidic linkage-containing glycolipids like ceramide-3 [galactosyl (α 1→4)galactosyl (ß 1→4)glucosyl ceramide) and digalactosyl ceramide. Measurement of the enzymic activity of this water-soluble enzyme can be performed using a water-soluble artificial substrate like p-nitrophenyl-α-galactoside. To measure the activity towards the water insoluble natural substrate a detergent is used. Micells between substrate and detergent are formed and make the glycolipid susceptible to enzymic degradation.Using crude and pure sodium taurocholate as detergent, we have investigated the factors influencing the enzymic activity of α-galactosidase A and the hydrolysis of ceramide-3.

Keywords

Artificial Substrate Sodium Taurocholate High Protein Concentration Detergent Concentration Hyperbolic Relation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • André W. Schram
    • 1
  • Mae Wan Ho
    • 2
  • Sylvia Cordus
    • 1
  • Marieke Samson
    • 1
  • Mic N. Hamers
    • 1
  • Jan Brown
    • 2
  • Don Robinson
    • 2
  • Joseph M. Tager
    • 1
  1. 1.Laboratory of BiochemistryUniversity of AmsterdamThe Netherlands
  2. 2.Department of BiochemistryQueen Elizabeth CollegeLondonEngland

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