Abstract
Complex lipids belonging to the group classified as “insoluble, swelling amphiphilic lipids” (1) form multibilayered liposomes when dispersed in aqueous media. These large aggregates are generally poor substrates for enzymatic utilization and, for interaction with suitable enzymes must be further dispersed or “solubilized”. This is usually done with the aid of a detergent (reviewed in ref. 2), although some success was obtained with sonicated, single-bilayered liposomes (e.g., 3). The exact nature of the effect, by the detergent that facilitates the interaction with the enzyme is still rather obscure. For numerous enzymatic reactions a nonionic detergent (e.g., Triton X-100) * or an anionic detergent (e.g., sodium dodecylsulfate or bile salts) was used; in some cases detergent mixtures were employed (2). It is generally assumed that the detergent exerts its beneficiary action by solubilizing the substrate. However, with some enzymes that utilize lipid substrates, a detergent enhanced reaction rates even when using a non-lipid, water-soluble substrate (e.g., (β-glucosidase using p-nitrophenyl glucoside (4) or methyl umbiliferyl glucoside (5). In such a case the detergent most probably affects the enzyme rather than the substrate. It is self-evident that if a lipid substrate (e.g., glucosyl-ceramide) is the substrate for the same enzyme, the detergent also solubilizes the substrate further to its affecting the enzyme.
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References
Small, D.M. (1970) Fed. Proc., 29, 1320–1326.
Gatt, S. (1973) in Metabolic Inhibitors, Vol. 4. Edited by Hochster, R. M., Kates, M. and Quastel, J.H. Academic Press, pp. 349–387.
Gatt, S. and Rapport, M. M. (1966) Biochim. Biophys. Acta 113, 567–576.
Gatt, S., Herzl, A. and Barenholz, Y. (1973) FEBS Letters 30, 281–285.
Ho, M.W. (1973) Biochem. J. 136, 721–729.
Gatt, S. and Gottesdiner, T. (1976), J. Neurochem. 26, 421–422.
Gatt, S. (1976) Biochem. Biophys. Res. Comm. 68, 235–241.
Gottesdiner, T. and Gatt, S. (1977) Israel J. Med. Sci., in press
Gatt, S., Dinur, T. and Leibovitz Ben-Gershon, Z., Submitted for publication.
Barenholz, Y., Rottman, A. and Gatt, S. (1966) J. Biol. Chem. 241, 3731–3737.
Cooper, G.V., Yedgar, S. and Barenholz, Y. (1974) Biochim. Biophys. Acta 363, 86–97.
Yedgar, S., Cooper, G.V., and Barenholz, Y. (1974) Ibid., pp. 98–111.
Lichtenberg, D., Yedgar, S. and Gatt, S., Submitted for publication.
Yedgar, S. and Gatt, S. (1976) Biochem. 15, 2570–2573.
Small, D.M. (1971) in The Bile Acids, Vol. 1. Edited by Nair, P.P. and Kritchevski, D., Plenum Press, New York, pp. 249–356.
Schneider, P.B. and Kennedy, E.P. (1967) J. Lipid Res. 8, 202209
Hirschfeld, D. and Loyter, A. (1975) Arch. Biochem. Biophys. 167, 186–192.
Rao, B.G. and Spence, M.W. (1970) J. Lipid Res. 17, 506–515.
Gatt, S. and Dinur, T. (1977) Proceedings of the 6th International Meeting of the International Society for Neurochemistry, Copenhagen, Aug. 21–26, in press.
Gatt, S., Dinur, T. and Kopolovic, submitted for publication.
Yamaguchi, S. and Suzuki, K. (1977) J. Biol. Chem., in press.
Gatt, S. (1966) J. Biol. Chem. 241, 37 24–3730.
Yavin, E. and Gatt, S. (1969) Biochem. 8, 1692–1698.
Sugita, M., Williams, M., Dulaney, J. T. and Moser, H.W. (1975), Biochim. Biophys. Acta, 398, 1 25–131.
Holloway, P.N. (1973) Anal. Biochem. 53, 304–308.
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Gatt, S., Dinur, T., Yedgar, S., Leibovitz-Ben Gershon, Z. (1978). Effect of Detergents on the Utilization of Sphingomyelin by Brain Sphingomyelinases. In: Gatt, S., Freysz, L., Mandel, P. (eds) Enzymes of Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 101. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9071-2_44
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DOI: https://doi.org/10.1007/978-1-4615-9071-2_44
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