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Studies on Bovine Brain Membrane-Bound Neuraminidase (Sialidase)

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 101)

Abstract

Mammalian neuraminidases have been localised inside the lysosomes, in the cytosol as well as in the various membranes of the cell cytocavitary system (1). Brain tissue is especially rich in membrane-bound neuraminidase which reaches its highest specific activity in the plasma membranes isolated from synaptosomes (2, 3). This enzyme cleaves terminal N-acylneuraminic acid residues, predominantly from oligosialogangliosides, thereby degrading them to monosialogangliosides (4–5).

Keywords

  • Nitrous Oxide
  • Sialic Acid
  • Water Soluble Substrate
  • Membrane Particle
  • Neuraminidase Activity

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Abbreviations

Des-GM1 :

(monosialo-gangliotetraitol): Galßl→3GalNAcßl→4Ga1[3←2αNeuAc]ß1→4[3H]sorbitol

Des-GD1a :

(disialo-gangliotetraitol): Gal [3←2αNeuAc] ß1→3GalNAcß 1→4Gal (3←2αNeuAc)ß1→4[3H]sorbitol

GA2 :

Gangliotriaosylceramide (GgOse3Cer): Ga1NACß1→4Galß1→4Glc1→l’ceramide

GM1 :

(ganglioside II3 NeuAc GgOse4-Cer): Galßl→3GaiNACß1→4Ga1[3←2αNeuAc]ß1→4G1cß1→1’ceramide

GM2 :

(ganglioside II3 NeuAc-GgOse3-Cer): Ga1NAcß1→4Gal [3←2αNeuAc]ß1→4Glcß1→1’ceramide

GDIa :

(ganglioside IV3 NeuAc, II3 NeuAc-GgOse4-Cer): Gal [3←2αNeuAc]ß1→3GalNAcß1→4Gal [3←2αNeuAc]ß1→4Glcß1→1’ceramide

NeuAc:

N-Acetylneuraminic acid

Sialyl-lactitol::

NeuAcα2→3Ga1ß1→4[3H]sorbitol

References

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© 1978 Plenum Press, New York

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Sandhoff, K., Pallmann, B., Wiegandt, H., Ziegler, W. (1978). Studies on Bovine Brain Membrane-Bound Neuraminidase (Sialidase). In: Gatt, S., Freysz, L., Mandel, P. (eds) Enzymes of Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 101. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9071-2_42

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  • DOI: https://doi.org/10.1007/978-1-4615-9071-2_42

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