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Interaction of Human Brain Neuraminidase with Tritium—Labelled Gangliosides

  • R. W. Veh
  • R. Schauer
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 101)

Abstract

Since the first demonstration of neuraminidase activity in mammals (1,2), the brain was recognised as a major source of this enzyme. In this organ soluble activity could be detected (3,4), however in most species the bulk of the enzyme proved to be membrane-bound. The first detailed description of a mammalian brain neuraminidase was given by Leibovitz and Gatt (5). Working with calf brain these investigators were able to solubilise about 30% of the neuraminidase activity by sequential treatment with sodium cholate and Triton X-100, and they obtained by this procedure an enzyme preparation purified sixfold over the starting material. Attempts to further purify the enzyme were unsuccessful. A modification of this method has been used in a detailed study of the neuraminidase present in human brain by Öhman et al. (3), but also in this case no further purification could be achieved.

Keywords

Galacturonic Acid Sodium Cholate Neuraminic Acid Prolonged Incubation Time Neuraminidase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1).
    WARREN, L., AND SPEARING, C.W., (1960) Mammalian Sialidase (Neuraminidase), Biochem. Biophys. Res. Commun. 3, 489–492CrossRefGoogle Scholar
  2. 2).
    CARUBELLI, R., TRUCCO, R.E., AND CAPUTTO, R. (1962) Neuraminidase Activity in Mammalian Organs, Biochim. Biophys. Acta 60, 196–197CrossRefGoogle Scholar
  3. 3).
    ÖHMAN, R., ROSENBERG, A., AND SVENNERHOLM, L. (1970) Human Brain Sialidase, Biochemistry 9, 3774–3782PubMedCrossRefGoogle Scholar
  4. 4).
    VENERANDO, B., TETTAMANTI, G., CESTARO, B., AND ZAMBOTTI, V. (1975) Studies on Brain Cytosol Neuraminidase. I. Isolation and Partial Characterization of Two Forms of the Enzyme from Pig Brain, Biochim. Biophys. Acta 403, 461–472CrossRefGoogle Scholar
  5. 5).
    LEIBOVITZ, Z., AND GATT, S. (1968) Enzymatic Hydrolysis of Sphingolipids. VII. Hydrolysis of Gang-liosides by a Neuraminidase from Calf Brain, Biochim. Biophys. Acta 152, 136–143CrossRefGoogle Scholar
  6. 6).
    SVENNERHOLM, L. (1963). Chromatographic Separation of Human Brain Gangliosides, J. Neurochem. 10, 613–623PubMedCrossRefGoogle Scholar
  7. 7).
    DRZENIEK, R. (1972) Viral and Bacterial Neuraminidases, in Current Topics in Micro-biology and Immunology, Vol. 59, pp. 35–74, Springer—Verlag, Berlin—Heidelberg—New YorkCrossRefGoogle Scholar
  8. 8).
    WARREN, L. (1959) The Thiobarbituric Acid Assay of Sialic Acids, J. Biol. Chem. 234, 1971–1975PubMedGoogle Scholar
  9. 9).
    AMINOFF, D. (1961) Methods for the Quantitative Estimation of N—Acetylneuraminic Acid and their Application to Hydrolysates of Sialomucoids, Biochem. J. 81, 384–392Google Scholar
  10. 10).
    VEH, R.W., SANDER, M., PARKER, T.L., AND SCHAUER, R. manuscript in preparationGoogle Scholar
  11. 11).
    SCHAUER, R., VEH, R.W., WEMBER, M., AND BUSCHER, H.P. (1976) Demonstration of Neuraminidase Activity in Human Blood Serum and Human Milk Using a Modified, Radioactively Labelled a1—Glycoprotein as Substrate, Hoppe—Seyler’s Z. Physiol. Chem. 357, 559–566CrossRefGoogle Scholar
  12. 12).
    VEH, R.W., CORFIELD, A.P., SANDER, M., AND SCHAUER, R. (1977) Neuraminic Acid—Specific Modification and Tritium Labelling of Gangliosides, Biochim. Biophys. Acta 486, 145–160CrossRefGoogle Scholar
  13. 13).
    TALLMAN, J.F., AND BRADY, R.O. (1973) The Purification and Properties of a Mammalian Neuraminidase (Sialidase), Biochim. Biophys. Acta 293, 434–443CrossRefGoogle Scholar
  14. 14).
    BHAVANANDAN, U.P., YEH, A.K., AND CARUBELLI, R. (1975) Neuraminidase Assay Utilizing Sialyl—Oligosaccharide Substrates with Tritium—Labeled Aglycone. Anal. Biochem. 69, 385–394PubMedCrossRefGoogle Scholar
  15. 15).
    STRECKER, G., MICHALSKI, J.C., MONTREUIL, J., AND FARRIAUX, J.P. (1976) Deficit in Neuraminidase Associated with Mucolipidosis II (I—Cell Disease), Biomedicine 25, 238–239PubMedGoogle Scholar
  16. 16).
    SUTTAJIT, M., AND WINZLER, R.J. (1971) Effect of Modification of N—Acetylneuraminic Acid on the Binding of Glycoproteins to Influenza Virus and on Susceptibility to Cleavage by Neuraminidase, J. Biol. Chem. 246, 3398–3404PubMedGoogle Scholar
  17. 17).
    WALOP, J.N., BOSCHMAN, Th.A.C., AND JACOBS, J. (1960) Affinity of N—Acetylneuraminic Acid for Influenza Virus Neuraminidase, Biochim. Biophys. Acta 44, 185–186CrossRefGoogle Scholar
  18. 18).
    MOHR, E. (1960) Hemmung der Neuraminidase aus Vibrio Cholerae, Z. Naturforsch. 15b, 575–577Google Scholar
  19. 19).
    GOTTSCHALK, A., AND DRZENIEK, R. (1972) Neuraminidase as a Tool in Structural Analysis, in Glycoproteins, Their Composition, Structure and Function ( Gottschalk, A., Ed.) pp. 381–402, Elsevier Publishing Company, AmsterdamLondon—New YorkGoogle Scholar
  20. 20).
    TETTAMANTI, G., VENERANDO, B., CESTARO, B., AND PRETI, A. (1976) Brain Neuraminidases and Gangliosides, in Ganglioside Function, Biochemical and Pharmacological Implications, ( Porcellati, G., Ceccarelli, B., and Tettamanti, G., eds.), pp. 65–79, Plenum Press, New York and LondonGoogle Scholar
  21. 21).
    HOFSTEE, B.H.J. (1959) Non—Inverted Versus Inverted Plots in Enzyme Kinetics, Nature 184, 1296–1298PubMedCrossRefGoogle Scholar
  22. 22).
    TULSIANI, D.R.P., AND CARUBELLI, R. (1970) Studies on the Soluble and Lysosomal Neuraminidases of Rat Liver, J. Biol. Chem. 245, 1821–1827PubMedGoogle Scholar
  23. 23).
    VISSER, A., AND EMMELOT, P. (1973) Studies on Plasma Membranes. XX. Sialidase in Hepatic Plasma Membranes, 3. Membrane Biol. 14, 73–84CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • R. W. Veh
    • 1
  • R. Schauer
    • 1
  1. 1.Institut für Physiologische ChemieRuhr-Universität BochumBochum 1Germany

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