Interaction of Human Brain Neuraminidase with Tritium—Labelled Gangliosides
Since the first demonstration of neuraminidase activity in mammals (1,2), the brain was recognised as a major source of this enzyme. In this organ soluble activity could be detected (3,4), however in most species the bulk of the enzyme proved to be membrane-bound. The first detailed description of a mammalian brain neuraminidase was given by Leibovitz and Gatt (5). Working with calf brain these investigators were able to solubilise about 30% of the neuraminidase activity by sequential treatment with sodium cholate and Triton X-100, and they obtained by this procedure an enzyme preparation purified sixfold over the starting material. Attempts to further purify the enzyme were unsuccessful. A modification of this method has been used in a detailed study of the neuraminidase present in human brain by Öhman et al. (3), but also in this case no further purification could be achieved.
KeywordsGalacturonic Acid Sodium Cholate Neuraminic Acid Prolonged Incubation Time Neuraminidase Activity
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- 9).AMINOFF, D. (1961) Methods for the Quantitative Estimation of N—Acetylneuraminic Acid and their Application to Hydrolysates of Sialomucoids, Biochem. J. 81, 384–392Google Scholar
- 10).VEH, R.W., SANDER, M., PARKER, T.L., AND SCHAUER, R. manuscript in preparationGoogle Scholar
- 18).MOHR, E. (1960) Hemmung der Neuraminidase aus Vibrio Cholerae, Z. Naturforsch. 15b, 575–577Google Scholar
- 19).GOTTSCHALK, A., AND DRZENIEK, R. (1972) Neuraminidase as a Tool in Structural Analysis, in Glycoproteins, Their Composition, Structure and Function ( Gottschalk, A., Ed.) pp. 381–402, Elsevier Publishing Company, AmsterdamLondon—New YorkGoogle Scholar
- 20).TETTAMANTI, G., VENERANDO, B., CESTARO, B., AND PRETI, A. (1976) Brain Neuraminidases and Gangliosides, in Ganglioside Function, Biochemical and Pharmacological Implications, ( Porcellati, G., Ceccarelli, B., and Tettamanti, G., eds.), pp. 65–79, Plenum Press, New York and LondonGoogle Scholar