Action of Rat Brain Sialidase on Synaptic Membrane Components in Situ
To provide further information on the characteristics of synaptic membrane sialidase, leading to some understanding of its biological role, we have examined the susceptibility of intrinsic sialo compounds of the synaptosomal membrane to the action of synaptic membrane sialidase in situ. Sialo compounds were labeled in vivo by the intracranial injection of radioactive N-acetylmannosamine during rapid synaptogenesis in rats (1) which is marked by ganglioside synthesis (2) and appearance of electrophysiological function (3). The high levels of sialo compounds and sialidase in mammalian nerve ending membranes lend importance to an understanding of their metabolism. Specific radioactive labeling has permitted sensitive and accurate observation of the behavior of intrinsic synaptic membrane sialidase and the various sialic acid-containing components.
KeywordsSialic Acid Barbituric Acid Synaptic Membrane Thin Layer Chromatogram Synaptosomal Membrane
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