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Ethanolamine and Choline Phosphotransferases of Chicken Brain

  • L. Freysz
  • L. A. Horrocks
  • P. Mandel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 101)

Abstract

Phospholipids are found almost exclusively in cellular membranes. Ethanolamine phosphoglycerides and choline phosphoglycerides are quantitatively the most important. They are in a constant state of metabolic flux with the rates of their synthesis and degradation often varying with the physiological activity of the tissues. Therefore the cells are continuously synthesizing new molecules of ethanolamine and choline phosphoglycerides. In brain, the last step of the de novo synthesis of these phosphoglycerides is carried out by CDP ethanolamine:1 ,2-diradyl-sn-glycerol ethanolamine phosphotransferase (EC 2.7.8.1) and CDP choline:1 ,2-diradyl-sn-glycerol phosphotransferase (EC 2.7.8.2) which transfer phosphoethanolamine or phosphocholine from the corresponding cytidine nucleotide to a diglyceride (1). Diglycerides are therefore situated at a branch point. They can be converted into ethanolamine phosphoglycerides, choline phosphoglycerides, tri-glycerides, or phosphatidic acids. Moreover CDP ethanolamine and CDP choline are also situated at a branch point since diacylphosphoglycerides are formed from diacylglycerols (2–4), alkenylacylphosphoglycerides from alkenylacylglycerols (5,6) and alkylacylphosphoglycerides from alkylacylglycerols (3,4,7,8). Thus the phosphotransferases are situated at branch points for both substrates. Since branch points are generally sites of enzyme regulation, we have studied the influence of possible effectors on the incorporation of different specific diglycerides and the utilization of CDP ethanolamine or CDP choline for ethanolamine and choline phosphoglyceride synthesis.

Keywords

Critical Micelle Concen Lipid Substrate Embryonic Life Chicken Brain Brain Microsome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • L. Freysz
    • 1
    • 2
  • L. A. Horrocks
    • 1
    • 2
  • P. Mandel
    • 1
    • 2
  1. 1.Centre de Neurochimie du CNRSStrasbourg CedexFrance
  2. 2.Department of Physiological ChemistryThe Ohio State University, ColumbusUSA

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