The Release of Free Ethanolamine in Rat Brain Homogenates Incubated in Krebs Ringer
Ethanolamine in mammalian brain is found chiefly in a lipid-bound form either as the diacyl phospholipid, phosphatidylethanolamine or as the plasmalogen, l-alk-l -enyl-2-acyl glycerophosphoethanolamine and to a lesser extent as the saturated ether analogue. The level of free ethanolamine in brain is very low, probably less than 40 nmol/g brain (Spanner & Ansell, 1977b) while that of phosphoethanolamine is about 1.0 μmol/g brain. Some time ago we found that if brain tissue was incubated in Krebs Ringer bicarbonate (pH 7.4) at 37°, there was a steady release of free ethanolamine by the tissue. The following account is a summary of the findings from experiments designed to determine the source of the ethanolamine liberated.
KeywordsBrain Homogenate Specific Radioactivity Intracerebral Injection Krebs Ringer Bicarbonate Steady Release
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