The Release of Free Ethanolamine in Rat Brain Homogenates Incubated in Krebs Ringer
- 90 Downloads
Ethanolamine in mammalian brain is found chiefly in a lipid-bound form either as the diacyl phospholipid, phosphatidylethanolamine or as the plasmalogen, l-alk-l -enyl-2-acyl glycerophosphoethanolamine and to a lesser extent as the saturated ether analogue. The level of free ethanolamine in brain is very low, probably less than 40 nmol/g brain (Spanner & Ansell, 1977b) while that of phosphoethanolamine is about 1.0 μmol/g brain. Some time ago we found that if brain tissue was incubated in Krebs Ringer bicarbonate (pH 7.4) at 37°, there was a steady release of free ethanolamine by the tissue. The following account is a summary of the findings from experiments designed to determine the source of the ethanolamine liberated.
KeywordsBrain Homogenate Specific Radioactivity Intracerebral Injection Krebs Ringer Bicarbonate Steady Release
Unable to display preview. Download preview PDF.