Summary
In the present investigation we have shown that cytoplasmic membranes of adipocytes contain Al and A2 phospholipase activities which are optimal in a buffer with 5 mM Ca 2+ (pH 8.5). Insulin enhances these activities within phosphatidylethanolamine. Insulin increases also the amount of free fatty acids in membranes.
Rodbell and Blecher have already shown an insulin-like action of phospholipases towards the uptake of glucose and amino-acids by adipocytes. Shier and Asakawa have recently described that lysolecithin and unsaturated fatty acids can change nucleotide-cyclase activities of cytoplasmic membranes towards GTP and ATP; lysolecithins and Triton X 100 seem to react in an identical way.
Results from these studies give new suggestions on insulin action; phospholipase activation changes membrane physiochemical properties inducing an increase of glucose carrier mobility and leading the membrane cyclase enzyme (s) towards GMP cyclic synthesis.
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© 1978 Plenum Press, New York
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Bereziat, G., Wolf, C., Colard, O., Polonovski, J. (1978). Phospholipases of Plasmic Membranes of Adipose Tissue. Possible Intermediaries for Insulin Action.. In: Gatt, S., Freysz, L., Mandel, P. (eds) Enzymes of Lipid Metabolism. Advances in Experimental Medicine and Biology, vol 101. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9071-2_18
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DOI: https://doi.org/10.1007/978-1-4615-9071-2_18
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