Chemical Modification of Crotalus Atrox Phospholipase A2 by Means of a Photolabile Phosphatidylethanolamine Analog
Although sequences have been reported for a number of pancreatic and venom phospholipase A2 enzymes, our knowledge of the interaction of these enzymes with their substrates is still very meager. There is general agreement (1,2) that a histidine residue plays a role in the catalytic reaction, but how it functions and what is the detailed course of the hydrolysis remains obscure. The necessary structural features for a compound to be a substrate have been known for some time (3,4), but we do not understand which of these features is involved in the protein-lipid interaction.
KeywordsSubstrate Analog Photolysis Product Medium Pressure Mercury Lamp Venom Phospholipase Active Site Histidine
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