Affinity Chromatography on Heparin-Sepharose of Rat Adipose Tissue Triglyceride Lipase from Cytosol
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Subfractionation of the fat free homogenate of rat adipose tissue showed that a high yield of triglyceride lipase was recovered reproducibly in the microsomal supernatant fraction (cytosol) when rat epididymal fat pads were homogenized in sucrose-EDTA-Tris medium.
Triglyceride lipase was bound on heparin-Sepharose. Hydrolyzing activity towards triacylglycerol was eluted as a single, sharp peak in 0.7 M NaCl, 5 mM sodium barbital and 20 % glycerol (pH 7.0). The triglyceride lipase was not inhibited by 1 M NaC1 and not stimulated by the presence of fresh human serum.
A lipoprotein-lipase activity was demonstrable in the cytosol when adipose tissue from fed rats were used. Fasting of the animals lowered this activity.
KeywordsLipase Activity Cyanogen Bromide Triglyceride Lipase Triacylglycerol Lipase Sodium Barbital
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