Abstract
The important requirements of an intracellular pH microelectrode are:
-
A.
A sharp tip to minimize cell wall damage on penetration.
-
C.
A smooth transition between the pH sensing portion and the insulated portion.
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D.
A conical angle as small as possible for the portion of the microelectrode that will be in the cell wall. A small angle will make a better microelectrode to cell wall seal thereby preventing leakage of intracellular fluid and shunting of the microelectrode (1).
-
E.
A fast and reproducible response to pH and a design that can be easily cleaned and rejuvenated.
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© 1974 Plenum Press, New York
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Hebert, N.C. (1974). Glass Microelectrodes for pH. In: Berman, H.J., Hebert, N.C. (eds) Ion-Selective Microelectrodes. Advances in Experimental Medicine and Biology, vol 50. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9023-1_3
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DOI: https://doi.org/10.1007/978-1-4615-9023-1_3
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