Skip to main content

Microelectrode Recording of Ion Activity in Brain

  • Chapter
Ion-Selective Microelectrodes

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 50))

Abstract

This paper deals with some technical aspects of the registration of extracellular potassium (K+) activity in the cortex of rats during hypoxia either by means of single-barrelled microelectrodes utilizing K+-sensitive liquid ion-exchanger (Corning Catalog no. 477317) (Cornwall et al., 1970; Khuri, 1971; Walker, 1971, 1973) or by means of double-barrelled microelectrodes, where one barrel measures electrical potential (the DC-barrel) and the other barrel measures electrical potential plus a potential determined by the K+-activity. (Vyskocil et al., 1972). We used a different method of producing double-barrelled electrodes from that described by Khuri et al., (1972a; 1972b) in order to obtain long shanks (5–10mm).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bradbury, M.W.B. Potassium Homeostasis in Cerebrospinal Fluid, p. 138 in Alfred Benzon Symposium III. Eds. Siesjo, B.K. and Sorenson, S.C. Munksgaard, Copenhagen, 1971.

    Google Scholar 

  • Cornwall, M.C., Peterson, D.F., Kunze, D.L., Walker, J.L. and Brown, A.M. Intracellular potassium and chloride activities measured with liquid ion exchanger microelectrodes. Brain Res. 1970, 23, 433.

    Article  PubMed  CAS  Google Scholar 

  • Engbak, E. and Guld, C. in Proceedings of the Second Nordic Meeting on Medical and Biological Engineering. June, 16–19, 1971, Oslo.

    Google Scholar 

  • Fenn, W.O. and Gerschmann, W. The Loss of Potassium from Frog Nerves in Anoxia and other Conditions. J. Gen. Physiol. 1949, 33, 195.

    Article  Google Scholar 

  • Frank, K. and Becker, M.C. Microelectrodes for Recording and Stimulation. In “Physical techniques in biological research” Vol. v, pp. 23–89. Ed. Nastuk, W.L., Academic Press, New York, 1964.

    Google Scholar 

  • Grossman, R.G. and Williams, V.F. Electrical Activity and Ultra-structure of Cortical Neurones and Synapses in Ischaemia. In “Brain Hypoxia”, p. 61. Eds. Brierly, J.B. and Meldrum, B.S. S.I.M.P./William Heineman. S.I.M.P./Medical Books Ltd., London, 1971.

    Google Scholar 

  • Hunyar, A., “Chemie der Silicone”, Verlag Technik, Berlin, 1959.

    Google Scholar 

  • Kolmodin, G.M. and Skogland, C.R. Influence of Asphyxia on Membrane Potential Level and Action Potentials of Spinal Moto-and Inter-neurones. Acta Physiol. Scand., 1959, 45, 1.

    Article  PubMed  CAS  Google Scholar 

  • Khuri, R.N. Intracellular Potassium and the Electrochemical Properties of Striated Muscle Fibers (Abstract). Proc, Intern. Union Physiol. Sci., 1971, 9, 301.

    Google Scholar 

  • Khuri, R.N., Aguilian, S.K. and Wise, W.M. Potassium in the rat kidney proximal tubules in situ: determination by K+-selective liquid ion-exchanger microelectrodes. Pflug. Arch, Ges. Physiol. 1971, 322, 39.

    CAS  Google Scholar 

  • Khuri, R.N., Aguilian, S.K. and Kalloghilan, A. Intracellular Potassium in Cells of the Distal Tubule. Pflug. Arch. Ges. Physiol. 1972a, 335, 297.

    Article  CAS  Google Scholar 

  • Khuri, R.N., Hagfar, J.J. and Aguilian, S.K. Measurement of intracellular potassium with liquid ion-exchange microelectrodes. J. Appl. Physiol., 1972b, 32, 419.

    PubMed  CAS  Google Scholar 

  • Kunze, D.L. and Brown, A.M. Internal Potassium and Chloride activities and the effects of acetylcholine on identifiable Aplysia neurones. Nature, 1971, 229, 229.

    CAS  Google Scholar 

  • Leao, A.A.P. Spreading depression of activity in the cerebral cortex, J. Neurophysiol. 1944, 7, 359.

    Google Scholar 

  • Sandblom, J.P., Eisenman, G. and Walker, J.L., Jr. Electrical Phenomenon associated with the Transport of Ions and Ion Pairs in Liquid Ion Exchanger Membranes. I. Zero Current Properties. II. Non-Zero Current Methods. J. Phys. Chem. 1967a, b, 71, 3862 and

    Article  PubMed  CAS  Google Scholar 

  • Sandblom, J.P., Eisenman, G. and Walker, J.L., Jr. Electrical Phenomenon associated with the Transport of Ions and Ion Pairs in Liquid Ion Exchanger Membranes. I. Zero Current Properties. II. Non-Zero Current Methods. J. Phys. Chem. 1967a, b, 71, 3871.

    Article  CAS  Google Scholar 

  • Thomas, R.C. New Design for Sodium-Sensitive Glass Microelectrodes J. Physiol. 1970, 210, 82P.

    Google Scholar 

  • Thomas, R.C. pNa Microelectrodes with Sensitive Glass Inside the Tip. In “Ion Selective Microelectrode”. Ed Hebert, N.C. and Khuri, R., Dekker, New York (1973) in Press.

    Google Scholar 

  • Walker, J.L., Jr. Ion Specific Liquid Ion Exchanger Microelectrodes Anal. Chem., 1971, 43, 89A.

    Article  CAS  Google Scholar 

  • Walker, J.L., Jr. Liquid Ion-Exchanger Microelectrodes for Ca2+”, CI” and K+. In “Ion Selective Microelectrodes” eds. Hebert, N.C. and Khuri, R.N., Dekker, New York, 1973 (in Press).

    Google Scholar 

  • Vyskocil, F., Kriz, N. and Bures, J. Potassium-selective microelectrodes used for measuring the extracellular brain potassium during spreading depression and anoxic depolarization in rats. Brain Research. 1972, 39, 255.

    Article  PubMed  CAS  Google Scholar 

  • Zeuthen, T., A Method to Fill Glass Microelectrodes by Local Heating. Acta physiol. Scand. 1971, 81, 141.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1974 Plenum Press, New York

About this chapter

Cite this chapter

Zeuthen, T., Hiam, R.C., Silver, I.A. (1974). Microelectrode Recording of Ion Activity in Brain. In: Berman, H.J., Hebert, N.C. (eds) Ion-Selective Microelectrodes. Advances in Experimental Medicine and Biology, vol 50. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9023-1_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-9023-1_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9025-5

  • Online ISBN: 978-1-4615-9023-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics