Abstract
The studies to be described involve the use of antisera directed against two alloantigens found on the surface of mouse lymphoid cells. Theta (θ) is found in brain as well as on thymocytes (1) and has recently been shown to be present on thymus-derived peripheral lymphocytes (2, 3, 4, 5). It can thus serve as a marker to distinguish thymus-derived lymphocytes from thymus-independent lymphocytes in peripheral lymphoid tissues. The thymus-leukemia antigen (TL) is normally found only on thymocytes in TL-positive strains of mice (6). We have used ∞ and TL as markers to study the development of thymocytes from embryonic stem cells, the differentiation of thymocytes to thymus-derived lymphocytes, and the emigration of thymus-derived lymphocytes to peripheral lymphoid tissues. 51chromium and dye-exclusion cytotoxic testing has been used to detect these alloantigens.
This work was supported in part by a Post-doctoral Fellowship from the National Multiple Sclerosis Society of the United States.
Work carried out while on leave of absence at the National Institute for Medical Research, Mill Hill.
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References
A. E. Reif and J. M. V. Allen, J. Exp. Med., 120:413, 1964
M. C. Raff, Nature (London), 224:378, 1969.
M. Schlesinger and I. Yron, Science, 164:1412, 1969.
M. C. Raff and H. H. Wortis, Immunology, 18:929, 1970.
M. Schlesinger and I. Yron, J. Immunol., 104:798, 1970.
T. Aoki, U. Hämmerling, E. de Harven, E. A. Boyse, and L. J. Old, J. Exp. Med., 130:979, 1969.
M. A. S. Moore and J. J. T. Owen, J. Exp. Med., 126:715, 1967.
J. J. T. Owen and M. A. Ritter, J. Exp. Med., 129:431, 1969.
J. J. T. Owen and M. C. Raff (submitted to J. Exp. Med.)
C. E. Ford, in: Ciba Found. Symp. Thymus: Experimental and Clinical Studies, G. E. W. Wolstenholme, Ed., p. 131. London: J. A. Churchill, Ltd., 1966.
M. Matsuyama, M. Wiadrowski, and D. Metcalf, J. Exp. Med., 123:559, 1966.
J. F. A. P. Miller, Proc. Roy. Soc., B, 156:415, 1962.
I. Weissman, J. Exp. Med., 126:291, 1967.
A. J. S. Davies, Transplant. Rev., 1:43, 1969.
M. C. Raff, Nature (London) (in press).
E. Leckband, Fed. Proc, 29:621 (Abs.), 1970.
H. Blomgren and B. Anderson, Exp. Cell Res., 57:185, 1969.
M. C. Raff and J. J. T. Owen (submitted to Europ. J. Immunol.)
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Raff, M.C., Owen, J.J.T. (1971). The Use of Surface Alloantigenic Markers to Study the Differentiation of Thymus-Derived Lymphocytes in Mice. In: Lindahl-Kiessling, K., Alm, G., Hanna, M.G. (eds) Morphological and Functional Aspects of Immunity. Advances in Experimental Medicine and Biology, vol 12. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9011-8_2
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