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Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte Membranes

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Book cover Methods in Membrane Biology

Abstract

Each biological membrane operates differentially on the two compartments it separates and is thus anisotropic in its function. It is reasonable to suppose that the molecular constituents of the two surfaces differ and that this asymmetry constitutes a principal feature of membrane organization. Since membranes are only a few macromolecules thick, it is clear that enumeration of the components at each surface would do much to define the structure as a whole.

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Abbreviations

RO:

right-side-out

IO:

inside-out

PBS:

150 mM NaCl-5 mM Na phosphate (pH 8.0)

5P8:

5 mM Na phosphate (pH 8.0)

0.5P8:

0.5 mM Na phosphate (pH 8.0)

AChase:

acetylcholinesterase (acetylcholine hydrolase, E.C. 3.1.1.7)

G3PD:

glyceraldehyde-3-phosphate dehydrogenase (d-glyceraldehyde-3-phosphate

NAD+ :

oxidoreductase [phosphorylating], E.G. 1.2.1.12)

NADH diaphorase:

reduced-NAD: (acceptor) oxidoreductase (E.C. 1.6.99.3)

DTNB:

5,5′-dithiobis-(2-nitrobenzoic acid).

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Authors and Affiliations

  1. Departments of Biochemistry and Medicine, University of Chicago, Chicago, Illinois, USA

    Theodore L. Steck

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  1. Theodore L. Steck
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Editor information

Editors and Affiliations

  1. Section on Cellular Biochemistry and Ultrastructure, National Heart and Lung Institute, Bethesda, Maryland, USA

    Edward D. Korn

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© 1974 Plenum Press, New York

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Steck, T.L. (1974). Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte Membranes. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8960-0_4

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  • DOI: https://doi.org/10.1007/978-1-4615-8960-0_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-8962-4

  • Online ISBN: 978-1-4615-8960-0

  • eBook Packages: Springer Book Archive

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