Abstract
Antibodies produced by inbred mouse strains immunized with the random synthetic polypeptide poly(Tyr, Glu)-poly(DLAla)—polyLys denoted (T, G)-A—L were found to be specific mainly to the ordered peptide Tyr-Tyr-Glu-Glu.
Low responder H-2k mice, upon immunization with either the random (T, G)-A—L or the ordered (T-T-G-G)-A—L coupled to methylated bovine serum albumin (MBSA), produce antibodies with comparable titers to those observed in high responder H-2b mice following immunization with the antigens alone or with their complexes with MBSA. A comparison of the above antibodies have led to the conclusion that low responder mice, upon immunization with the synthetic antigens complexed with MBSA, produce antibodies of the same specificity and quality as those of high responders (as shown by the isoelectric focusing technique) and they also have the same affinity and heterogeneity as antibodies of H-2° mice (measured by equilibrium dialysis and antigen binding capacity assay).
Anti-idiotypic sera to anti-(T, G)-A—L antibodies of C3H.SW (H-2b, Ig-la) mice were raised in guinea pigs. C3H.SW anti-(T, G)-A—L antibodies from different pools cross reacted idiotypically. Anti-(T, G)-A—L antibodies of CWB (H-2b, Ig-lb) mice did not react with the anti-idiotypic serum suggesting linkage between the genes coding for idiotypes and allotypes. C3H/DiSn (H-2k, Ig-la) anti-(T, G)-A—L antibodies elicited by immunization with (T, G)-A—L complexed to MBSA reacted with the anti-idiotypic serum to the same degree as C3H.SW anti-(T, G)-A—L antibodies, confirming the similarity between the high and low responder anti-(T, G)-A—L antibodies.
C3H.SW (H-2b) mice as well as C3H/HeJ or C3H/DiSn (H-2k) mice were found to be capable of producing an antigen specific factor from “educated” T cells which replaces the helper effect of T cells in the process of antibody production. On the other hand B cells of H-2 mice were not triggered by a factor of either high or low responder specific T cells.
The activity of a C3H.SW (T, G)-A—L specific T cell factor was removed after passage on a Sepharose column coupled to the antiidiotypc serum prepared against C3H.SW anti-(T, G)-A—L antibodies, suggesting similarity between the antigen specific T cell factor and the B cell recognition system. A (T, G)-A—L specific factor produced by C3H/DiSn (H-2k, Ig-la) “educated” T cells reacted with the anti-idiotypic serum as well. Thus, C3H.SW high and C3H/DiSn low responder (T, G)-A—L specific T cell factors cross react at the level of their binding site for antigen.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Binz, H., and Wigzell, H. (1975) J. Exp. Med. 142, 197.
Claman, H.N., Chaperon, E.A., and Triplett, R.F. (1966) J. Immunol. 97, 828.
Cosenza, H., and Köhler, H. (1972) Proc. Natl. Acad. Sci. USA 69, 2701.
Cramer, M., Schwartz, M., Mozes, E., and Sela, M. (1976) Eur. J. Immunol. 6, 618.
Eichmann, K. (1972) Eur. J. Immunol. 2, 301.
Eichmann, K., and Kindt, T.J. (1971) J. Exp. Med. 134, 532.
Eichmann, K., and Rajewsky, K. (1975) Eur. J. Immunol. 5, 661.
Fathman, G.G., and Sachs, D.H. (1976) J. Immunol. 116, 959.
Isac, R., and Mozes, E. (1977) J. Immunol. 118, 584.
Isac, R., Dorf, M.E., and Mozes, E. (1977) Immunogenetics, in press.
Kuettner, M.G., Wang, A.L. and Nisonoff, A. (1972) J. Exp. Med. 135, 579.
Lichtenberg, L., Mozes, E., Shearer, G.M., and Sela, M. (1974) Eur. J. Immunol. 4, 430.
McDevitt, H.O. (1968) J. Immunol. 100, 485.
McDevitt, H.O., and Chinitz, A. (1969) Science 163, 1207.
McDevitt, H.O., and Sela, M. (1965) J. Exp. Med. 122, 517.
McDevitt, H.O., and Tyan, M.L. (1968) J. Exp. Med. 128, 1.
Mitchell, G.F., and Miller, J.F.A.P. (1968) J. Exp. Med. 128, 821.
Mitchison, N.A. (1971) Eur. J. Immunol. 1, 10.
Mozes, E. (1976) in “The Role of the Products of the Histocompatibility Gene Complex in Immune Responses” (D.H. Katz and B. Benacerraf, Eds.) p. 485. Academic Press, New York.
Mozes, E. (1977) in Proceedings of the 3rd Ir gene workshop. (H.O. McDevitt, Ed.) Academic Press, New York, in press.
Mozes, E., and Shearer, G.M. (1972). Curr. Top. Microbiol. Immunol. 59, 167.
Mozes, E., Schwartz, M., and Sela, M. (1974) J. Exp. Med. 140, 349.
Mozes, E., Isac, R., Givol, D., Zakut, R., and Beitsch, D. (1975) in “Immune Reactivity of Lymphocytes” (M. Feldman and A. Globerson, Eds.) Adv. Exp. Med. Biol. 66, 397. Plenum Press, New York.
Nisonoff, A., Hopper, J.E., and Spring, S.B. (1975) The antibody molecule. Academic Press, New York.
Ramachandran, J., Berger, A., and Katchalski, E. (1971) Biopolymers 10, 1829.
Schwartz, M., Mozes, E., and Sela, M. (1975) Eur. J. Immunol. 5, 866.
Sela, M., Fuchs, S., and Arnon, R. (1962) Biochem. J. 85, 223.
Steward, M.N., and Petty, R.E. (1972) Immunology 23, 881.
Taussig, M.J. (1974) Nature 248, 234.
Taussig, M.J., and Munro, A.J. (1974) Nature 251, 63.
Taussig, M.J., Mozes, E., and Isac, R. (1974) J. Exp. Med. 140, 301.
Taussig, M.J., Munro, A.J., Campbell, R., David, C.S., and Staines, N.A. (1975) J. Exp. Med. 142, 694.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1978 Plenum Press, New York
About this chapter
Cite this chapter
Mozes, E. (1978). The Nature and Functions of Specific Immune Response Genes and their Products. In: Atassi, M.Z., Stavitsky, A.B. (eds) Immunobiology of Proteins and Peptides · I. Advances in Experimental Medicine and Biology, vol 98. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8858-0_25
Download citation
DOI: https://doi.org/10.1007/978-1-4615-8858-0_25
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-8860-3
Online ISBN: 978-1-4615-8858-0
eBook Packages: Springer Book Archive