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Release of Hypothalamic Hormones under in Vivo and in Vitro Conditions

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Book cover Hypothalamic Peptide Hormones and Pituitary Regulation

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 87))

Abstract

The presence in hypothalamic tissue of extractable substances, called releasing factors or hormones, which can either stimulate or repress the release of hormones from the adenohypophysis is well established. Moreover, the activities of these hypothalamic substances have been demonstrated under in vivo as well as in vitro conditions. At least three of these factors, luteinizing hormone releasing hormone (LHRH), thyrotropin releasing hormone (TRH), and somatostatin, have been isolated in pure form and found on analysis to be small peptides (1–4). Their amino acid sequences have been determined, and each has been synthesized. The availability of these synthetic compounds has made it possible to develop sensitive immunoassays for each peptide. As a consequence, it is now feasible to address critically the issue of secretion of these hypothalamic hormones and to test with rigor the question of whether these peptides have significant roles in the physiological regulation of the adenohypophysis.

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References

  1. Burgus, R., T. F. Dunn, D. Desiderio, and R. Guillemin, “Structure moléculaire du facteur hypothalamique hypophysiotrope TRF d’origine ovine: Mise en évidence par spectrométrie de masse de la séquence PCA-His-Pro-NH2,” C R Acad Sci [D] (Paris) 269: 1870–1873, 1969.

    CAS  Google Scholar 

  2. Schally, A. V., A. Arimura, I. Wakabayashi, S. Sawano, J. F. Barrett, C. Y. Bowers, T. W. Redding, J. C. Mittler, and M. Saito, “The chemistry of hypothalamic growth hormone-releasing hormone (GRH),” Hypophysiotropic Hormones of the Hypothalamus: Assay and Chemistry, J. Meites (ed.), Williams and Wilkins, Baltimore, 1970, pp. 208–226.

    Google Scholar 

  3. Matsuo, H., Y. Baba, R. M. G. Nair, A. Arimura, and A. V. Schally, “Structure of the porcine LH-and FSH-releasing hormone. I. The proposed amino acid sequence,” Biochem Biophys Res Commun 43: 1334–1339, 1971.

    Article  PubMed  CAS  Google Scholar 

  4. Brazeau, P., W. Vale, R. Burgus, N. Ling, M. Butcher, J. Rivier, and R. Guillemin, “Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone,” Science 179: 77–79, 1973.

    Article  PubMed  CAS  Google Scholar 

  5. Cramer, O. M., and C. A. Barraclough, “Effect of electrical stimulation of the preoptic area on plasma LH concentrations in proestrous rats,” Endocrinology 88: 1175–1183, 1971.

    Article  PubMed  CAS  Google Scholar 

  6. Clemens, J. A., C. J. Shaar, J. W. Kleber, and W. A. Tandy, “Areas of the brain stimulatory to LH and FSH secretion,” Endocrinology 88: 180–184, 1971.

    Article  PubMed  CAS  Google Scholar 

  7. Kalra, S. P., K. Ajika, L. Krulich, C. P. Fawcett, M. Quijada, and S. M. McCann, “Effects of hypothalamic and preoptic electrochemical stimulation on gonadotropin and prolactin release in proestrous rats,” Endocrinology 88: 1150–1158, 1971.

    Article  PubMed  CAS  Google Scholar 

  8. Turgeon, J., and C. A. Barraclough, “Temporal patterns of LH release following graded preoptic electrochemical stimulation in proestrous rats,” Endocrinology 92: 755–761, 1973.

    Article  PubMed  CAS  Google Scholar 

  9. Eskay, R. L., R. S. Mical, and J. C. Porter, “Relationship between luteinizing hormone releasing hormone concentration in hypophysial portal blood and luteinizing hormone release in intact, castrated, and electrochemically-stimulated rats,” Endocrinology 100: 263–270, 1977.

    Article  PubMed  CAS  Google Scholar 

  10. Eskay, R. L., C. Oliver, N. Ben-Jonathan, and J. C. Porter, “Hypothalamic hormones in portal and systemic blood,” Hypothalamic Hormones, M. Motta, P. G. Crosignani, and L. Martini (eds.), Academic Press, New York, 1975, p. 125–137.

    Google Scholar 

  11. Oliver, C., N. Ben-Jonathan, R. S. Mical, and J. C. Porter, “Transport of thyrotropin-releasing hormone from cerebrospinal fluid to hypophysial portal blood and the release of thyrotropin,” Endocrinology 97: 1138–1143, 1975.

    Article  PubMed  CAS  Google Scholar 

  12. Coyle, J. T., and D. Henry, “Catecholamines in fetal and newborn rat brain,” J Neurochem 21: 61–67, 1973.

    Article  PubMed  CAS  Google Scholar 

  13. Ben-Jonathan, N., and J. C. Porter, “A sensitive radioenzymatic assay for dopamine, norepinephrine, and epinephrine in plasma and tissue,” Endocrinology 98: 1497–1507, 1976.

    Article  PubMed  CAS  Google Scholar 

  14. Ben-Jonathan, N., C. Oliver, H. J. Weiner, R. S. Mical, and J. C. Porter, “Dopamine in hypophysial portal plasma of the rat during the estrous cycle and throughout pregnancy,” Endocrinology 100: 452–458, 1977.

    Article  PubMed  CAS  Google Scholar 

  15. Fuxe, K., T. Hökfelt, and O. Nilsson, “Activity changes in the tubero-infundibular dopamine neurons of the rat during various states of the reproductive cycle,” Life Sci 6: 2057–2061, 1967.

    Article  PubMed  CAS  Google Scholar 

  16. Björklund, A., R. Y. Moore, A. Nobin, and U. Stenevi, “The organization of turbero-hypophyseal and reticulo-infundibular catecholamine neuron systems in the rat brain,” Brain Res 51: 171–191, 1973.

    Article  PubMed  Google Scholar 

  17. MacLeod, R. M., “Influence of norepinephrine and catecholamine-depleting agents on the synthesis and release of prolactin and growth hormone,” Endocrinology 85: 916–923, 1969.

    Article  PubMed  CAS  Google Scholar 

  18. Birge, C. A., L. S. Jacobs, C. T. Hammer, and W. H. Daughaday, “Catecholamine inhibition of prolactin secretion by isolated rat adenohypophyses,” Endocrinology 86: 120–130, 1970.

    Article  PubMed  CAS  Google Scholar 

  19. Shaar, C. J., and J. A. Clemens, “The role of catecholamines in the release of anterior pituitary prolactin in vitro,” Endocrinology 95: 1202–1212, 1974.

    Article  PubMed  CAS  Google Scholar 

  20. Whittaker, V. P., “The synaptosome,” Handboodk of Neurochemistry, vol II, A. Lajtha (ed.), Plenum Press, New York, 1969, pp. 327–364.

    Google Scholar 

  21. De Robertis, E., and G. Rodriguez de Lores Arnaiz, “Structural components of the synaptic region,” Handbook of Neurochemistry, vol II, A. Lajtha (ed.), Plenum Press, New York, 1969, pp. 365–392.

    Google Scholar 

  22. Jones, D. G., Synapses and Synaptosomes, Morphological Aspects, Chapman and Hall, London, 1975.

    Google Scholar 

  23. Barnea, A., N. Ben-Jonathan, C. Colston, J. M. Johnston, and J. C. Porter, “Differential sub-cellular compartmentalization of thyrotropin releasing hormone (TRH) and gonadotropin releasing hormone (LRH) in hypothalamic tissue,” Proc Natl Acad Sci USA 72: 3153–3157, 1975.

    Article  PubMed  CAS  Google Scholar 

  24. Barnea, A., N. Ben-Jonathan, and J. C. Porter, “Characterization of hypothalamic subcellular particles containing luteinizing hormone releasing hormone and thyrotropin releasing hormone,” J. Neurochem 27: 477–484, 1976.

    Article  PubMed  CAS  Google Scholar 

  25. Bennett, G. W., J. A. Edwardson, D. Holland, S. L. Jeffcoate, and N. White, “Release of immunoreactive luteinising hormone-releasing hormone and thyrotropin-releasing hormone from hypothalamic synaptosomes,” Nature 257: 323–325, 1975.

    Article  PubMed  CAS  Google Scholar 

  26. Warberg, J., R. L. Eskay, A. Barnea, R. C. Reynolds, and J. C. Porter, “Release of luteinizing hormone releasing hormone and thyrotropin releasing hormone from a synaptosome-enriched fraction of hypothalamic homogenates,” Endocrinology 100: 814–825, 1977.

    Article  PubMed  CAS  Google Scholar 

  27. Eskay, R. L., J. Warberg, R. S. Mical, and J. C. Porter, “Prostaglandin E2-induced release of LHRH into hypophysial portal blood,” Endocrinology 97: 816–824, 1975.

    Article  PubMed  CAS  Google Scholar 

  28. Green, J. D., and G. W. Harris, “The neurovascular link between the neurohypophysis and adenohypophysis,” J. Endocrinol 5: 136–146, 1947.

    Article  PubMed  CAS  Google Scholar 

  29. Török, B., “Lebendbeobachtung des hypophysenkreislaufes an hunden,” Acta Morphol Acad Sci Hung 4: 83–89, 1954.

    PubMed  Google Scholar 

  30. Török, B., “Neue angaben zum blutkreislauf der hypophyse,” Anat Anz 109: 622–629, 1960.

    Google Scholar 

  31. Szentágothai, J., B. Flerkô, B. Mess, and B. Halász, Hypothalamic Control of the Anterior Pituitary, ed. 3, Akademiai Kiado, Budapest, 1968, p. 81.

    Google Scholar 

  32. Jazdowska, B., and W. Dobrowolski, “Vascularization of the hypophysis in sheep,” Endokrynol Pol 16: 269–282, 1965.

    PubMed  CAS  Google Scholar 

  33. Page, R. B., B. L. Munger, and R. M. Bergland, “Scanning microscopy of pituitary vascular casts,” Amer J Anat 146: 273–302, 1976.

    Article  PubMed  CAS  Google Scholar 

  34. Landsmeer, J. M. F., “Vessels of the ratTs hypophysis,” Acta Anat 12: 82–109, 1951.

    Article  PubMed  CAS  Google Scholar 

  35. Page, R., and R. Bergland, “Vascular organization within the mammalian hypophysis. Part I: median eminence,” V Internatl Congr Endocrinol, Hamburg, Germany, 1976, p. 206 (Abstract no. 502).

    Google Scholar 

  36. Bergland, R., and R. Page, “Vascular organization within the mammalian hypophysis. Part II: interrelationships,” V Internatl Congr Endocrinol, Hamberg, Germany, 1976, p. 206 (Abstract no. 503).

    Google Scholar 

  37. Oliver, C., R. S. Mical, and J. C. Porter, “Hypothalamic-pituitary vasculature: Evidence for retrograde blood flow in the pituitary stalk,” Endocrinology, August 1977 (in press).

    Google Scholar 

  38. Porter, J. C., and K. R. Smith, “Collection of hypophysial stalk blood in rats,” Endocrinology 81: 1182–1185, 1967.

    Article  PubMed  CAS  Google Scholar 

  39. Porter, J. C., R. S. Mical, I. A. Kamberi, and Y. R. Grazia, “A procedure for the cannulation of a pituitary stalk portal vessel and perfusion of the pars distalis in the rat,” Endocrinology 87: 197–201, 1970.

    Article  PubMed  CAS  Google Scholar 

  40. Ben-Jonathan, N., R. S. Mical, and J. C. Porter, “Superfusion of hemipituitaries with portal blood. I. LRF secretion in castrated and diestrous rats,” Endocrinology 93: 497–503, 1973.

    Article  PubMed  CAS  Google Scholar 

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Author information

Author notes

  1. Robert L. Eskay

    Present address: National Institute for Alcohol Abuse and Alcoholism, St. Elizabeth’s Hospital, Washington, D. C., USA

  2. Charles Oliver

    Present address: Labortoire de Médecine Experimentale, U.E.R. Médecine Nord, Boulevard Pierre Dramard, Marseille, 13326, France

  3. Nira Ben-Jonathan

    Present address: Department of Physiology, Indiana University Medical School, Indianapolis, Indiana, USA

  4. Jørgen Warberg

    Present address: Institute of Medical Physiology C, University of Copenhagen, Copenhagen, Denmark

Authors and Affiliations

  1. Cecil H. & Ida Green Center for Reproductive Biology Sciences Departments of Obstetrics & Gynecology and Physiology, The University of Texas Health Science Center at Dallas Southwestern Medical School, Dallas, Texas, 75235, USA

    John C. Porter, Robert L. Eskay, Charles Oliver, Nira Ben-Jonathan, Jørgen Warberg, C. Richard Parker Jr. & Ayalla Barnea

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  1. John C. Porter
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  2. Robert L. Eskay
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  4. Nira Ben-Jonathan
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  6. C. Richard Parker Jr.
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  7. Ayalla Barnea
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Editor information

Editors and Affiliations

  1. University of Texas Health Science Center at Dallas, USA

    John C. Porter

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© 1977 Plenum Press, New York

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Porter, J.C. et al. (1977). Release of Hypothalamic Hormones under in Vivo and in Vitro Conditions. In: Porter, J.C. (eds) Hypothalamic Peptide Hormones and Pituitary Regulation. Advances in Experimental Medicine and Biology, vol 87. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8849-8_9

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  • DOI: https://doi.org/10.1007/978-1-4615-8849-8_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-8851-1

  • Online ISBN: 978-1-4615-8849-8

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