Abstract
Recent literature has shown that the thin-layer chromatographic-enzyme inhibition (TLC-EI) technique is a promising and practical method for analysis of pesticide residues, metabolites, or enzyme inhibitors. Various enzymatic methods for pesticide residue analysis were reviewed by Devlin (1959), Archer (1963), Guilbault (1966 and 1970 a), and Zweig and Moore (1970). Guilbault (1970 a) and Zweig and Moore (1970) also reviewed the use of the TLC-EI technique for the detection of pesticides. Enzymatic methods of analysis have been extensively treated in a book by Guilbault (1970 b).
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References
Abbott, D. C., K. W. Blake, K. R. Tarrant, and J. Thomson: Thin-layer chromatographic separation, identification and estimation of residues of some carbamate and allied pesticides in soil and water. J. Chromatog. 30, 136 (1967).
Abbott, D. C., and J. Thomson: Pesticide residue analysis by thin-layer chromatography. Pest Articles and New Summaries, Sect. A. (Insect Control) 12, 21 (1966).
Ackermann H.: Enzymatischer Nachweis phosphororganischer Insektizide nach diinnschichtchromatographischer Trennung. Nahrung 10, 273 (1966).
Ackermann H.: Dünnschichtchromatographisch-enzymatischer Nachweis phosphororganischer Insektizide: Aktivierung schwacher Esterasehemmer. J. Chromatog. 36, 309 (1968).
Ackermann H.: Untersuchungen zur Hemmung der Cholinesterase durch Thionophosphorsäureester. Arch. Toxikol. 24, 325 (1969).
Ackermann H., and R. Engst: Vorkommen von phosphororganischen Insecticiden im Fetus. Arch. Toxikol. 26, 17 (1970).
Archer, T. E.: Enzymatic methods. In G. Zweig (ed.): Analytical methods for pesticides, plant growth regulators, and food additives, Vol. 1, p. 373. New York: Academic Press (1963).
Beitz, H., and M. Ehrt: Zum Nachweis von Thio-and Dithiophosphorsäureestern sowie Esterasehemmern auf Diinnschichtchromatogrammen. Z. Chem. (Leipzig) 8, 387 (1968).
Beroza, M., K. R. Hill, and K. H. Norris: Determination of reflectance of pesticide spots on thin-layer chromatograms using fiber optics. Anal. Chem. 40, 1608 (1968).
Beynon, K. I., and M. J. Edwards: Residues of tetrachlorvinphos and its breakdown products on treated crops. III. Residues on a range of field crops. Pesticide Sci. 1, 259 (1970).
Bunyan, P. J.: The detection of organophosphorus pesticides on thin-layer chromatograms. Analyst 89, 615 (1964).
Caster, W. O., and J. W. Andrews, Jr.: An optical integrator for quantitative measurement in thin-layer chromatography. J. Chromatog. Sci. 7, 408 (1969).
Coox, J. W.: Paper chromatography of some organic phosphate insecticides. IV. Spot test for in vitro cholinesterase inhibitors. J. Assoc. Official Agr. Chemists 38, 150 (1955).
Crosby, D. G., E. Lerrls, and W. L. Winterlin: Photodecomposition of carbamate insecticides. J. Agr. Food Chem. 13, 204 (1965).
Crossley, J.: GLC and TLC determination of tetraethyl pyrophosphate (TEPP) in crops. J. Assoc. Official Anal. Chemists 53, 1036 (1970).
Dallas, M. S. J.: The effect of layer thickness on Rf values in thin-layer chromatography. J. Chromatog. 33, 193 (1968).
De Klein, W. J.: Analysis of spots obtained from thin-layer chromatography by gas-liquid chromatography using a total-spot injection technique. Fresenius Z. Anal. Chem. 249, 81 (1970).
De Thomas, A. V., Zdankiewicz, and D. Witkovic: A modified “sandwich” microapparatus for descending thin-layer chromatography. Microchem. J. 15, 71 (1970).
Devlin, T. M.: Enzymatic methods in analytical chemistry. Anal. Chem. 31, 977 (1959).
De Zeeuw, H., Compaan, F. J. Ritter, J. H. Dhont, C. Vinkenborg, and R. P. Labadie: Improved separation in thin-layer chromatography using a single-component solvent in an unsaturated chamber. J. Chromatog. 47, 382 (1970).
Dhont, J. H., and C. de Roo: Thin-layer chromatography of 3,5-dinitrobenzoates. Analyst 86, 527 (1961).
Dhont, J. H., C. Vinkenborg, H. Compaan, F. J. Ritter, R. P. Labadie, A. Verweij, and R. A. de Zeeuw: Application of Rf correction in thin-layer chromatog- raphy by means of two reference Rf values. J. Chromatog. 47, 376 (1970).
Elrefai, A., and T. L. Hopkins: Thin-layer chromatography and cholinesterase detection of several phosphorothiono insecticides and their oxygen analogs. J. Agr. Food Chem. 13, 477 (1965).
Fechner, G., H. Ackermann, and B. Lexow: Diinnschichtchromatographische Bestimmung von Dimethoate und PO-Dimethoate in Milch. Z. Lebensm. Untersuch. u. Forsch. 140, 145 (1969 a).
Fechner, G., and H. Toepfer: Bestimmung von DDVP in Luft. Fresenius Z. Anal. Chem. 246, 259 (1969 b).
Galanos, D. S., and V. M. Kapoulas: The paper chromatographic identification of compounds using two reference compounds. J. Chromatog. 13, 128 (1964).
Geike, F.: Diinnschichtchromatographisch-enzymatischer Nachweis und zum Wirkungsmechanismus von Chlorkohlenwasserstoff-Insektiziden. J. Chromatog. 44, 95 (1969).
Geike, F.: Dünnschichtchromatographisch-enzymatischer Nachweis und zum Wirkungsmechanismus von Chlorkohlenwasserstoff-Insektiziden. II. Nachweis durch Hemmung von Trypsin. J. Chromatog. 52, 447 (1970 a).
Geike, F.: Insektizide-und Antiesterase-Wirkung von Chlorkohlenwasserstoff-Insektiziden nach UV-Bestrahlung. Z. Angewandte Entomol. 65, 98 (1970 b).
Geike, F.: Dünnschichtchromatographisch-enzymatischer Nachweis von Carbamaten. I. Nachweis insektiziden Carbamate mit Rinderleber-Esterase. J. Chromatog. 53, 269 (1970 c).
Geike, F.: Diinnschichtchromatographisch-enzymatischer und gaschromatographischer Nachweis von 4,4’-Dichlorbenzophenon und seinen Abbauprodukten. J. Chromatog. 54, 282 (1971).
Getz, M. E., and S. J. Friedman: Organophosphate pesticide residues: A spot test for detecting cholinesterase inhibitors. J. Assoc. Official Agr. Chemists 46, 707 (1963).
Goldman:, J., and R. R. Goodall: Quantitative analysis on thin layer chromatograms: A theory for light absorption methods with an experimental verifica- tion. J. Chromatog. 32, 24 (1968).
Goldman:, J., and R. R. Goodall: Quantitative analysis on thin layer chromatograms: Application of an optical theory to in situ analysis. J. Chromatog. 40, 345 (1969).
Goldman:, J., and R. R. Goodall: Quantitative analysis on thin-layer chromatograms: An instrument and method for in situ analysis using ultraviolet light. J. Chromatog. 47, 386 (1970).
Grant, D. L., C. R. Sherwood, and K. A. Mccully: Gas-liquid and thin-layer chromatography of phorate, disulfoton and five of their oxidation products. J. Chromatog. 44, 67 (1969).
Guilbault, G. G.: Use of enzymes in analytical chemistry. Anal. Chem. 38, 527R (1966).
Guilbault, G. G.: Use of enzymes in and kinetic aspects of analytical chemistry. Anal. Chem. 42, 33411 (1970 a).
Guilbault, G. G.: Enzymatic methods of analysis. Oxford and Toronto: Pergamon (1970 b).
Haahti, E., and I. Jaakonmaki: A new monitoring principle and apparatus for quantitative detection of solutes in thin-layer chromatograms. Ann. Med. Exp. Fenn. 47, 175 (1969).
Haahti, E., R. Vihko, I. Jaakonmaki, and R. S. Evans: Quantitative detection of solutes in thin-layer chromatography. J. Chromatog. Sci. 8, 370 (1970).
Hill, K. R.: Pesticide analysis by thin-layer chromatography. Chem. Speciality Mfg. Assoc. Proc. 55, 172 (1968).
Honegger, C. G.: Neuere Untersuchungen zur präparativen Diinnschichtchromatographie. Hely. Chim. Acta 46, 1772 (1963).
Hopkins, T. L.: In vivo oxidation of ronnel in the Madeira cockroach. J. Econ. Entomol. 55, 334 (1962).
Hopkins, T. L., and F. W. Knapp: Anticholinesterases in blood and cattle grubs from cattle treated with ronnel. J. Econ. Entomol. 56, 872 (1963).
Kenaga, E. E.: Commercial and experimental organic insecticides (1966 Revision). Bull. Entomol. Soc. Amer. 12, 161 (1966).
Kenaga, E. E., and W. E. Allison: Commercial and experimental organic insecticides (1969 Revision). Bull. Entomol. Soc. Amer. 15, 85 (1969).
Leelinc, N. C., and J. E. Casma: Metabolites of carbaryl (1-naphthyl methylcarbamate) in mammals and enzymatic systems for their formation. J. Agr. Food Chem. 14, 281 (1966).
Lefar, M. S., and A. D. Lewis: Thin-layer densitometry. Anal. Chem. 42, 79A (1970).
Mcicinley, W. P., and P. S. Jonal: Esterase inhibition technique for detection of organophosphorus pesticides. II. Simplified version for routine checking. J. Assoc. Official Agr. Chemists 46, 840 (1963).
Mcicinley, W. P., and S. I. Read: Esterase inhibition technique for the detection of organophosphorus pesticides. J. Assoc. Official Agr. Chemists 45, 467 (1962).
Mendoza, C. E., D. L. Grant, B. Braceland, and K. A. Mccully: Evaluation of esterases from livers of beef, pig, sheep, monkey and chicken for detection of some pesticides by thin-layer chromatographic-enzyme inhibition technique. Analyst 94, 805 (1969 b).
Mendoza, C. E., and J. B. Shields: Sensitivity of pig liver esterase in detecting twelve carbamate pesticides on thin-layer chromatograms. J. Chromatog. 50, 92 (1970).
Mendoza, C. E., and J. B. Shields: Esterase specificity and sensitivity to organophosphorus and carbamate pesticides: Factors affecting determination by thin-layer chromatography. J. Assoc. Official Anal. Chemists 54, 508 (1971).
Mendoza, C. E., P. J. Wales, and D. F. Bray: Consistency of R3, values of six organophosphorus pesticides resolved by thin-layer chromatography in the presence of plant extracts without elaborate clean-up. Analyst 93, 688 (1968 c).
Mendoza, C. E., D. L. Grant, and K. A. Mccully: Effect of bromine and ultraviolet light on eight pesticides detected with liver esterases of five species. J. Agr. Food Chem. 17, 1196 (1969 a).
Mendoza, C. E., and G. V. Hatina: Alkoxy derivatives of dyrene: Identification and carboxylesterase inhibition. J. Agr. Food Chem. 19, 41 (1971).
Mendoza, C. E., and H. A. Mcleod: Simultaneous detection of some organophosphorus pesticides in whole wheat flour. Bull. Environ. Contam. Toxicol. 5, 276 (1970).
Mendoza, C. E., and W. P. Mckinley: Enzymatic detection of ten organophosphorus pesticides and carbaryl on thin-layer chromatograms: An evaluation of indoxyl, substituted indoxyl and 1-naphthyl acetates as substrates of esterases. Analyst 93, 34 (1968 a).
Mendoza, C. E., and W. P. Mckinley: Thin-layer chromatographic-enzyme inhibition procedure to screen for organophosphorus pesticides in plant extracts without elaborate clean-up. Analyst 93, 173 (1968 b).
Menn, J. J., and J. B. McBain: Detection of cholinesterase-inhibiting insecticide chemicals and pharmaceutical alkaloids on thin-layer chromatograms. Nature 209, 1351 (1966).
Menn, J. J., and M. J. Dennis: Detection of naturally occurring cholinesterase inhibitors in several crops by paper chromatography. Nature 202, 697 (1964).
Morrison, R. T., and R. N. Boyd: Organic chemistry. Boston: Allyn and Bacon (1959).
Neumeyer, J., D. Gibbons, and H. Trask: CW report: Pesticides, Part I. Chem. Week, Apr. 12, p. 37 (1969 a).
CW report: Pesticides, Part II. Chem. Week, Apr. 26, p. 37 (1969 b).
Oonnithan, E. S., and J. E. Casida: Metabolites of methyl-and dimethylcarbamate insecticide chemicals as formed by rat liver microsomes. Bull. Environ. Contam. Toxicol. 1, 59 (1966).
Oonnithan, E. S., and J. E. Casida: Oxidation of methyl-and dimethylcarbamate insecticide chemicals by microsomal enzymes and anticholinesterase activity of the metabolites. J. Agr. Food Chem. 16, 28 (1968).
Ortloff, R., and P. Franz: Zwei neue Methoden der Biochemischen Lokalisierung von phosphorhaltigen Insektiziden auf Diinnschichtchormatogrammen. Z. Chem. (Leipzig) 5, 388 (1965).
Pataki, G., and M. Keller: Einfluss der Schichtdicke auf die R1-Werte in der Dünnschichtchromatographie. Heiv. Chim. Acta 46, 1054 (1963).
Perish, C. R.: A two-parameter formula relating R, values in different solvent systems. Anal. Chem. 40, 551 (1968).
Reichel, W. L.: Effects of humidity in the laboratory on thin-layer chromatography of insecticides. J. Chromatog. 26, 304 (1967).
Robbins, W. E., T. L. Hopkins, and D. I. Damow: Synergistic action of piperonyl butoxide with Bayer 21/199 and its corresponding phosphate in mice. J. Econ. Entomol. 52 660 (1959).
Schutzmann, R. L.: Note on improved spray reagents for TLC fluorogenic detection of cholinesterase inhibitors. J. Assoc. Official Anal. Chemists 53, 1056 (1970).
Schutzmann, R. L., and W. F. Barthel: Indoxyl acetate spray reagent for fluorogenic detection of cholinesterase inhibitors in environmental samples. J. Assoc. Official. Anal. Chemists 52, 151 (1969).
Severin, M.: Chromatographie sur couche mince des 3,5-dinitrobenzoates: Séparation des alcools aliphatiques de C, à C44 par chromatographie bidimensionnelle. J. Chromatog. 26, 101 (1967).
Spencer, E. Y.: Guide to the chemicals used in crop protection, 5th ed. Ottawa, Canada: Queen’s Printer (1968).
Wales, P. J., H. A. Mcleod, and W. P. Mckiniey: TLC-enzyme inhibition procedure to detect some carbamate standards and carbaryl in food extracts. J. Assoc. Official Anal. Chemists 51, 1239 (1968 b).
Wales, P. J., and C. E. Mendoza: Investigation on determination and confirmation of dyrene added to plant extracts: GLC and TLC of dyrene and products of its reaction in methanolic sodium hydroxide. J. Assoc. Official Anal. Chemists 53, 509 (1970).
Wales, P. J., H. A. McLeod, and W. P. Mckinley: Procedure for semiquantitative confirmation of some organophosphorus pesticide residues in plant extracts. Analyst 93, 691 (1968 a).
Winterlin, W., G. Walker, and H. Frank: Detection of cholinesterase-inhibiting pesticides following separation on thin-layer chromatograms. J. Agr. Food Chem. 16, 808 (1968).
Zweig, G., and R. B. Moore: Chromatography. Anal. Chem. 42, 349R (1970).
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Mendoza, C.E. (1972). Analysis of pesticides by the thin-layer chromatographic-enzyme inhibition technique. In: Gunther, F.A., Gunther, J.D. (eds) Residue Reviews. Residue Reviews / Rückstands-Berichte, vol 43. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-8485-8_4
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DOI: https://doi.org/10.1007/978-1-4615-8485-8_4
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