Abstract
In Drosophila melanogaster premutational lesions induced in mature spermatozoa lead to mutations only after insemination of the egg. This concept is supported by the following experimental evidence: (1) X-ray induced mutations in mature sperm do not show a dose fractionation effect (Muller, 1940). (2) The frequencies of sex-chromosome loss recovered from X-rayed sperm depend on the type of female used for the test cross; the females show maternal effects (Würgler and Maier, 1972). (3) The frequencies of sex-linked recessive lethals recovered from sperm mutagenized with chemical compounds are modified by the presence or absence of DNA repair systems in the oocytes into which the treated sperm are introduced for the test cross (Graf et al., 1979a; Würgler and Graf, 1980). (4) Sperm mutagenized with mono-functional alkylating agents give increased frequencies of sex chromosome loss when tested with excision repair deficient (mei-9 a) oocytes (Zimmering et al., 1981).
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Abbreviations
- CP:
-
cyclophosphamide (CAS number 50-18-0)
- EMS:
-
ethyl methanesulfonate (62-50-0)
- ENU:
-
nitrosoethylurea (759-73-9)
- HN2:
-
nitrogen mustard, methyl di (2-chloroethyl) amine (51-75-2)
- MMC:
-
mitomycin C (50-07-7)
- MMS:
-
methyl methanesulfonate (66-27-3)
- MNU:
-
nitrosomethylurea (684-93-5)
References
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© 1982 Plenum Press, New York
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Graf, U., Würgler, F.E. (1982). DNA Repair Dependent Mutagenesis in Drosophila Melanogaster. In: Lakovaara, S. (eds) Advances in Genetics, Development, and Evolution of Drosophila. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8321-9_9
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