Skip to main content

Liver Support Through Hepatic Tissue Engineering

  • Chapter
Tissue Engineering

Abstract

Tissue engineering is a relatively new field which brings together several disciplines, including bioengineering, molecular and cellular biology and clinical medicine, with the ultimate goal of producing tissue or organ substitutes. The spectrum of applications of tissue engineering is indeed wide from artificial skin substitutes (Bell et al., 1991) and transplantable cell-polymer matrices (Cima et al., 1991) to bioartificial organs such as the bioartificial liver and bioartificial pancreas (Yarmush et al.,1992a; 1992b; Colton and Avgoustiniatos, 1992). All of these technologies have a number of common aspects, such as (1) the identification of reliable and efficient sources of cells and isolation of cells from tissues, (2) means of culture and preservation of cells, and (3) tissue design.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Arnaout WS, Moscioni AD, Barbour RL, and Demetriou AA. (1990): Development of bioartificial liver: bilirubin conjugation in Gunn rats. J. Surg. Res. 48: 379–382.

    Article  Google Scholar 

  • Bader A, Borel Rinkes IHM, Class E, Ryan C, Toner M, Cunningham J, Tompkins RG, and Yarmush ML (1992): A stable long-term hepatocyte culture system for studies of physiologic processes: Cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotech Prog 8: 219–225.

    Article  Google Scholar 

  • Bell E, Rosenberg M, Kemp P, Gay R, Green GD, Muthukumaran N, and Nolte C (1991): Recepies for reconstituting skin. J. Biomech. Eng. 113: 113–119.

    Article  Google Scholar 

  • Berry MN, and Friends DS (1986): High-yiled preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43: 506–520.

    Article  Google Scholar 

  • Borel Rinkes IHM, Toner M, Ezzell RM, Tompkins RG, and Yarmush ML (1992a): Effect of dimethyl sulfoxide on cultured rat hepatocytes in sandwich configuration. Cryobiol 29: 443–453.

    Article  Google Scholar 

  • Borel Rinkes IHM, Toner M, Tompkins RG, and Yarmush ML (1992b): Longterm functional recovery of hepatocytes after cryopreservation in a three-dimensional culture configuration. Cell Transplantation 1: 281–292.

    Google Scholar 

  • Cima LG, Vacanti JP, Vacanti C, Ingber D, Mooney D, and Langer R (1991): Tissue engineering by cell transplantation using degradble polymer substrates. J. Biomech. Eng. 113: 143–151.

    Article  Google Scholar 

  • Colton CK, and Avgoustiniatos ES (1991): Bioengineering in development of the hybrid artificial pancreas. J. Biomech. Eng. 113: 151–170.

    Article  Google Scholar 

  • Dunn JCY, Yannush ML, Tompkins RG, and Koebe H (1989): Hepatocyte function and extracellular matrix geometry: Long-term culture in a sandwich configuration. FASEB J 3: 174–177.

    Google Scholar 

  • Dunn JCY (1991): Long-term culture of differentiated hepatocytes in a collagen sandwich configuration. Ph.D. Thesis, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA.

    Google Scholar 

  • Dunn JCY, Tompkins, RG, and Yarmush, MY (1991): Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotech Prog 7: 237–245.

    Article  Google Scholar 

  • Dunn J, Tompkins RG, and Yarmush ML (1992a): Hepatocytes in collagen sandwich configuration: Evidence for transcriptional and translational control. J Cell Biol 116: 1043–1053.

    Article  Google Scholar 

  • Dunn JCY, Friedberg JS, Tompkins RG, and Yarmush ML (1992b): Hepatocytes from rat liver perfusions: physicochemical effects on polyribosome size. ASAIO J. 38: 841–845.

    Google Scholar 

  • Dunn JCY, Yarmush MLY, and Tompkins RGT (1993, in press): Dynamics of transcriptional and translational processes in hepatocytes cultured in collagen sandwich. Biotech Bioeng.

    Google Scholar 

  • Eyre DR (1980): Collagen: molecular diversity in the body’s protein scaffold. Science 207: 1315–1322.

    Google Scholar 

  • Ezzell RM, Toner M, Hendricks K, Dunn JCY, Tompkins RG, and Yannush ML (in preparation): Dynamics and structure of cytoskeletal organization of cultured hepatocytes: effects of the collagen gel.

    Google Scholar 

  • Foy, BD, Lee, J, Morgan, J, Toner, M, Tompkins, RG, and Yarmush, ML (1993, in press): Optimization of hepatocyte attachment to microcarriers: importance of oxygen. Biotech and Bioeng.

    Google Scholar 

  • Harris CL, Toner M, Hubel A, Cravalho EG, Yarmush ML, and Tompkins RG (1991): Cryopreservation of isolated hepatocytes: intracellular ice formation under various physical and chemical conditions. Cryobiol 28: 17–30.

    Article  Google Scholar 

  • Hubel A, Toner M, Cravalho EV, Yarmush ML, and Tompkins RG (1991): Intracellular ice formation during the freezing of hepatocyte culture in a double collagen gel. Biotech Prog 7: 554–559.

    Article  Google Scholar 

  • Kleinman HK, Klebe RJ, and Martin GR (1981): Role of collagenous matrices in the adhesion and growth of cells. J. Cell Biol. 88: 473–485.

    Article  Google Scholar 

  • Knowles BB, Howe CC, and Aden DP (1980): Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigens. Science 209: 497–499.

    Article  Google Scholar 

  • Koebe H, Dunn JCY, Toner M, Sterling LM, Hubel A, Cravalho EG, Yarmush ML, and Tompkins RG (1990): A new approach to the cryopreservation of hepatocytes in a sandwich configuration. Ciyobiol 27: 587–594.

    Google Scholar 

  • Creamer BL, Staecker JL, Sawada GL, Sattler MT, Hsia MT, and Pitot H (1986): Use of a low-speed, iso-density percoll centrifugation method to increase viability of isolated rat hepatocytes preparations. In Vitro Cell. Del). Biol. 22: 201–211.

    Article  Google Scholar 

  • Lee J, Morgan JR, Tompkins RG, and Yarmush ML (1992): The importance of proline on long-term hepatocyte function in a collagen sandwich configuration: regulation of protein secretion. Biotech and Bioeng 40: 298–305.

    Article  Google Scholar 

  • Lee J, Morgan JR, Tompkins RG, and Yarmush ML (1993, in press): Proline mediated enhancement of hepatocyte function in a collagen gel sandwich culture configuration. FASEB J.

    Google Scholar 

  • Loree, HM (1988): A freeze-drying process for fabrication of polymeric bridges for peripheral nerve regeneration. M.S. degree thesis, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA.

    Google Scholar 

  • Mazur P (1984): Freezing of living cells: mechanisms and implications. Am. J. Physiol. 143: C125 - C142.

    Google Scholar 

  • Reese JA, and Byard JL (1981): Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 22: 935–940.

    Article  Google Scholar 

  • Rotem A, Toner M, Tompkins RG, and Yarmush ML (1992): Oxygen uptake rates in cultured rat hepatocytes. Biotech Bioeng 8: 227–232.

    Google Scholar 

  • Rotem A, Toner M, Bhatia S, Foy, BD, Tompkins RG, and Yarmush ML (in preparation): The effect of oxygen on attachment and spreading of cultured rat hepatocytes.

    Google Scholar 

  • Ryan C, Carter EA, Jenkins R, Sterling L, Yannush ML, Malt R, and Tompkins RG (1993): Isolation and long-term culture of human hepatocytes. Surgery 113: 48–54.

    Google Scholar 

  • Seglen PO (1976): Preparation of isolated rat liver cells. Methods Cell Biol. 13: 29–83.

    Article  Google Scholar 

  • Shnyra A, Bocharov A, Bochkova N, and Spirov V (1990): Large-scale production and cultivation of hepatocytes on Biosilon microcarriers. Artif. Organs 14: 421–428.

    Article  Google Scholar 

  • Sussman NL, Chong MG, Koussayer T, He D, Shang TA, Whisennad HH, and Kelly JH (1992): Reversal of fulminant hepatic failure using an extracorporeal liver assist device. Hepatology 16: 60–65.

    Article  Google Scholar 

  • Tompkins RG, Carter EA, Carlson J, and Yarmush ML (1988): Enzymatic function of alginate immobilized rat hepatocytes. Biotech Bioeng 31: 1118.

    Article  Google Scholar 

  • Toner M, Borel Rinkes IHM, Cravalho EG, Tompkins RG, and Yannush ML (1993, in press): A controlled rate freezing device for cryopreservation of biological tissue. Ciyo-Lett.

    Google Scholar 

  • Toner M, Cravalho EG, Tompkins RG, and Yarmush ML (1992): Transport phenomena during freezing of isolated hepatocytes. AIChE.1 38: 1512–1522.

    Article  Google Scholar 

  • Uchino J, Tsuburaya T, Kumagai F, Hase T, Hamada T, Kornai T, Funatsu A, Hashimura E, Nakamura K, and Kon T (1988): A hybrid bioartificial liver composed of multiplated hepatocyte monolayers. ASAIO Trans. 34: 972–977.

    Google Scholar 

  • Yarmush ML, Dunn J, and Tompkins RG (1992a): Assessment of artificial liver support technology. Cell Transplantation 1: 410–423.

    Google Scholar 

  • Yarmush ML,Toner M, Dunn JCY, Rotem A, Hubel A, and Tompkins RG (1992b): Hepatic tissue engineering: Development of critical technologies. Ann N.Y. Acad Sci 665: 472–485.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Birkhäuser Boston

About this chapter

Cite this chapter

Toner, M., Tompkins, R.G., Yarmush, M.L. (1993). Liver Support Through Hepatic Tissue Engineering. In: Bell, E. (eds) Tissue Engineering. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4615-8186-4_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-8186-4_9

  • Publisher Name: Birkhäuser, Boston, MA

  • Print ISBN: 978-1-4615-8188-8

  • Online ISBN: 978-1-4615-8186-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics