Abstract
Tissue engineering is a relatively new field which brings together several disciplines, including bioengineering, molecular and cellular biology and clinical medicine, with the ultimate goal of producing tissue or organ substitutes. The spectrum of applications of tissue engineering is indeed wide from artificial skin substitutes (Bell et al., 1991) and transplantable cell-polymer matrices (Cima et al., 1991) to bioartificial organs such as the bioartificial liver and bioartificial pancreas (Yarmush et al.,1992a; 1992b; Colton and Avgoustiniatos, 1992). All of these technologies have a number of common aspects, such as (1) the identification of reliable and efficient sources of cells and isolation of cells from tissues, (2) means of culture and preservation of cells, and (3) tissue design.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Arnaout WS, Moscioni AD, Barbour RL, and Demetriou AA. (1990): Development of bioartificial liver: bilirubin conjugation in Gunn rats. J. Surg. Res. 48: 379–382.
Bader A, Borel Rinkes IHM, Class E, Ryan C, Toner M, Cunningham J, Tompkins RG, and Yarmush ML (1992): A stable long-term hepatocyte culture system for studies of physiologic processes: Cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotech Prog 8: 219–225.
Bell E, Rosenberg M, Kemp P, Gay R, Green GD, Muthukumaran N, and Nolte C (1991): Recepies for reconstituting skin. J. Biomech. Eng. 113: 113–119.
Berry MN, and Friends DS (1986): High-yiled preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43: 506–520.
Borel Rinkes IHM, Toner M, Ezzell RM, Tompkins RG, and Yarmush ML (1992a): Effect of dimethyl sulfoxide on cultured rat hepatocytes in sandwich configuration. Cryobiol 29: 443–453.
Borel Rinkes IHM, Toner M, Tompkins RG, and Yarmush ML (1992b): Longterm functional recovery of hepatocytes after cryopreservation in a three-dimensional culture configuration. Cell Transplantation 1: 281–292.
Cima LG, Vacanti JP, Vacanti C, Ingber D, Mooney D, and Langer R (1991): Tissue engineering by cell transplantation using degradble polymer substrates. J. Biomech. Eng. 113: 143–151.
Colton CK, and Avgoustiniatos ES (1991): Bioengineering in development of the hybrid artificial pancreas. J. Biomech. Eng. 113: 151–170.
Dunn JCY, Yannush ML, Tompkins RG, and Koebe H (1989): Hepatocyte function and extracellular matrix geometry: Long-term culture in a sandwich configuration. FASEB J 3: 174–177.
Dunn JCY (1991): Long-term culture of differentiated hepatocytes in a collagen sandwich configuration. Ph.D. Thesis, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA.
Dunn JCY, Tompkins, RG, and Yarmush, MY (1991): Long-term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotech Prog 7: 237–245.
Dunn J, Tompkins RG, and Yarmush ML (1992a): Hepatocytes in collagen sandwich configuration: Evidence for transcriptional and translational control. J Cell Biol 116: 1043–1053.
Dunn JCY, Friedberg JS, Tompkins RG, and Yarmush ML (1992b): Hepatocytes from rat liver perfusions: physicochemical effects on polyribosome size. ASAIO J. 38: 841–845.
Dunn JCY, Yarmush MLY, and Tompkins RGT (1993, in press): Dynamics of transcriptional and translational processes in hepatocytes cultured in collagen sandwich. Biotech Bioeng.
Eyre DR (1980): Collagen: molecular diversity in the body’s protein scaffold. Science 207: 1315–1322.
Ezzell RM, Toner M, Hendricks K, Dunn JCY, Tompkins RG, and Yannush ML (in preparation): Dynamics and structure of cytoskeletal organization of cultured hepatocytes: effects of the collagen gel.
Foy, BD, Lee, J, Morgan, J, Toner, M, Tompkins, RG, and Yarmush, ML (1993, in press): Optimization of hepatocyte attachment to microcarriers: importance of oxygen. Biotech and Bioeng.
Harris CL, Toner M, Hubel A, Cravalho EG, Yarmush ML, and Tompkins RG (1991): Cryopreservation of isolated hepatocytes: intracellular ice formation under various physical and chemical conditions. Cryobiol 28: 17–30.
Hubel A, Toner M, Cravalho EV, Yarmush ML, and Tompkins RG (1991): Intracellular ice formation during the freezing of hepatocyte culture in a double collagen gel. Biotech Prog 7: 554–559.
Kleinman HK, Klebe RJ, and Martin GR (1981): Role of collagenous matrices in the adhesion and growth of cells. J. Cell Biol. 88: 473–485.
Knowles BB, Howe CC, and Aden DP (1980): Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigens. Science 209: 497–499.
Koebe H, Dunn JCY, Toner M, Sterling LM, Hubel A, Cravalho EG, Yarmush ML, and Tompkins RG (1990): A new approach to the cryopreservation of hepatocytes in a sandwich configuration. Ciyobiol 27: 587–594.
Creamer BL, Staecker JL, Sawada GL, Sattler MT, Hsia MT, and Pitot H (1986): Use of a low-speed, iso-density percoll centrifugation method to increase viability of isolated rat hepatocytes preparations. In Vitro Cell. Del). Biol. 22: 201–211.
Lee J, Morgan JR, Tompkins RG, and Yarmush ML (1992): The importance of proline on long-term hepatocyte function in a collagen sandwich configuration: regulation of protein secretion. Biotech and Bioeng 40: 298–305.
Lee J, Morgan JR, Tompkins RG, and Yarmush ML (1993, in press): Proline mediated enhancement of hepatocyte function in a collagen gel sandwich culture configuration. FASEB J.
Loree, HM (1988): A freeze-drying process for fabrication of polymeric bridges for peripheral nerve regeneration. M.S. degree thesis, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA.
Mazur P (1984): Freezing of living cells: mechanisms and implications. Am. J. Physiol. 143: C125 - C142.
Reese JA, and Byard JL (1981): Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 22: 935–940.
Rotem A, Toner M, Tompkins RG, and Yarmush ML (1992): Oxygen uptake rates in cultured rat hepatocytes. Biotech Bioeng 8: 227–232.
Rotem A, Toner M, Bhatia S, Foy, BD, Tompkins RG, and Yarmush ML (in preparation): The effect of oxygen on attachment and spreading of cultured rat hepatocytes.
Ryan C, Carter EA, Jenkins R, Sterling L, Yannush ML, Malt R, and Tompkins RG (1993): Isolation and long-term culture of human hepatocytes. Surgery 113: 48–54.
Seglen PO (1976): Preparation of isolated rat liver cells. Methods Cell Biol. 13: 29–83.
Shnyra A, Bocharov A, Bochkova N, and Spirov V (1990): Large-scale production and cultivation of hepatocytes on Biosilon microcarriers. Artif. Organs 14: 421–428.
Sussman NL, Chong MG, Koussayer T, He D, Shang TA, Whisennad HH, and Kelly JH (1992): Reversal of fulminant hepatic failure using an extracorporeal liver assist device. Hepatology 16: 60–65.
Tompkins RG, Carter EA, Carlson J, and Yarmush ML (1988): Enzymatic function of alginate immobilized rat hepatocytes. Biotech Bioeng 31: 1118.
Toner M, Borel Rinkes IHM, Cravalho EG, Tompkins RG, and Yannush ML (1993, in press): A controlled rate freezing device for cryopreservation of biological tissue. Ciyo-Lett.
Toner M, Cravalho EG, Tompkins RG, and Yarmush ML (1992): Transport phenomena during freezing of isolated hepatocytes. AIChE.1 38: 1512–1522.
Uchino J, Tsuburaya T, Kumagai F, Hase T, Hamada T, Kornai T, Funatsu A, Hashimura E, Nakamura K, and Kon T (1988): A hybrid bioartificial liver composed of multiplated hepatocyte monolayers. ASAIO Trans. 34: 972–977.
Yarmush ML, Dunn J, and Tompkins RG (1992a): Assessment of artificial liver support technology. Cell Transplantation 1: 410–423.
Yarmush ML,Toner M, Dunn JCY, Rotem A, Hubel A, and Tompkins RG (1992b): Hepatic tissue engineering: Development of critical technologies. Ann N.Y. Acad Sci 665: 472–485.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Birkhäuser Boston
About this chapter
Cite this chapter
Toner, M., Tompkins, R.G., Yarmush, M.L. (1993). Liver Support Through Hepatic Tissue Engineering. In: Bell, E. (eds) Tissue Engineering. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4615-8186-4_9
Download citation
DOI: https://doi.org/10.1007/978-1-4615-8186-4_9
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-1-4615-8188-8
Online ISBN: 978-1-4615-8186-4
eBook Packages: Springer Book Archive