Abstract
One of the major recurring themes in this symposium on Tissue Engineering is that to design effective artificial tissues, we must first understand the critical chemical and structural determinants that control tissue development. Current tissue engineering approaches commonly use cell attachment scaffolds that are complex composites of naturally occuring extracellular matrix (ECM) molecules (e.g., collagens, glycosaminoglycans). Unfortunately, these “artificial ECMs” are restricted from an engineering standpoint: they exhibit a limited range of structural and chemical properties and are not easily chemically modified. Also, their large-scale fabrication can be limited by “batch to batch” variability during purification of the individual ECM molecules. An alternative approach for cell transplantation is to develop a completely synthetic attachment foundation that can support a high degree of cell function and yet be highly biocompatible (Vacanti et al., 1988; Cima et al., 1991). To accomplish this objective, recent advances in ECM biology must be merged with new developments in bioengineering and polymer chemistry.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Albelda SM and Buck CA (1990): Integrins and other cell adhesion molecules. FASEB J 4: 2868–2880.
Bell E, Ivarsson B and Merrill C (1979): Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potentials in vitro. Proc Natl Acad Sci USA 76: 1274–1278.
Ben-Ze’ev A, Robinson GS, Bucher NL and Fariner SR (1988): Cell-cell and cell-matrix interactions differentially regulate the expression of hepatic and cytoskeletal genes in primary cultures of rat hepatocytes. Proc Nati Acad Sci USA 85: 1–6.
Bissell DM, Arenson DM, Maher JJ and Roll FJ (1987): Support of Cultured Hepatocytes by a Laminin-rich Gel. Am Soc Clin Inv 79: 801–812.
Buxbaum RE and Heidemann SR (1988): A thermodynamic model for force integration and microtubule assembly during axonal elongation. J Theor Biol 134: 379–390.
Cima LG, Vacanti JP, Vacanti C, Ingber DE, Mooney DJ and Langer RL (1991): Tissue Engineering by Cell Transplantation Using Biodegradable Polymers. J Biomech Eng 38: 145–158.
Dunn JC, Yarmush ML, Koebe HG and Tompkins RG (1989): Hepatocyte Function and Extracellular Matrix Geometry: Long-term culture in a Sandwich Configuration. FASEB J 3: 174–177.
Emerman JT and Pitelka DR (1977): Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In Vitro Cell Dev Biol 13: 316–328.
Erickson CA and Trinkaus JP (1976): Microvilli and blebs as sources of reserve surface membrane during cell spreading. Exp Cell Res 99: 375–384.
Feldherr C and Akin D (1990): The permeability of the nuclear envelope in dividing and nondividing cell cultures. J Cell Biol 98: 1973–1984.
Hansen LH, Mooney DJ and Ingber DE (1991): Clustering of integrins induces expression of the early growth response gene, junB, in hepatocytes, independent of cell spreading. J Cell Biol 115: 443a.
Hansen LK and Ingber DE (1992): Regulation of nucleocytoplasmic transport by mechanical forces transmitted through the cytoskeleton. In: Feldherr C, ed. Nuclear Trafficking, Academic Press: Orlando, FL pp. 71–86.
Harris AK (1982): Traction, and its relations to contraction in tissue cell locomotion. In: Cell Behavior, ( Bellairs, R., Curtis, A., Dunn, G., eds.) Cambridge University Press: Cambridge, pp. 109–134.
Hill TL (1981): Microfilament or microtubule assembly or disassembly against a force. Proc Natl Acad Sci USA 78: 5613–5617.
Ingber DE (1990): Fibronectin controls capillary endothelial cell growth by modulating cell shape. Proc Natl Acad Sci USA 87: 3579–3583.
Ingber DE (1991). Integrins as mechanochemical transducers. Curr Opin Cell Biol 3: 841–848.
Ingber DE (1993) Cellular tensegrity: defining new rules of biological design that govern the cytoskeleton. J Cell Sci in press.
Ingber DE and Folkman J (1989a): Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix. J Cell Biol 109: 317–330.
Ingber DE and Folkman J (1989b): How does extracellular matrix control capillary morphogenesis? Cell 58: 803–805.
Ingber DE and Folkman J (1989c) Tension and compression as basic determinants of cell form and function: utilization of a cellular tensegrity mechanism. In: Stein W, Bronner F, eds. Cell Shape: Determinants, Regulation and Regulatory Role. Academic Press: Orlando, pp. 1–32.
Ingber DE and Jamieson JD (1985): Cells as tensegrity structures: architectural regulation of histodifferentiation by physical forces tranduced over basement membrane. In: Andersson LC, Gahmberg CG, Ekblom P, eds. IN: Gene Expression During Normal and Malignant Differentiation. Academic Press: Orlando, pp. 13–32.
Ingber DE, Karp S, Plopper G, Hansen L and Mooney D (1993): Mechanochemical transduction across extracellular matrix and through the cytoskeleton. In: Frangos JA, Ives CL, eds. Physical Forces and the Mammalian Cell. Academic Press: San Diego. pp. 61–78.
Ingber DE, Madri JA and Folkman J (1987): Extracellular matrix regulates endothelial growth factor action through modulation of cell and nuclear expansion. In Vitro Cell Dey Biol 23: 387–394.
Ingber DE, Prusty, D, Frangione J, Cragoe EJ Jr, Lechene C and Schwartz M (1990): Control of intracellular pH and growth by fibronectin in capillary endothelial cells. J Cell Biol 110: 1803–1812.
Koch J (1917): The laws of bone architecture. Am JAnat 21: 177–198.
Kreis TE and Birchmeier W (1980): Stress fiber sarcomeres of fibroblasts are contractile. Cell 22: 555–561.
Li ML, Aggeler J, Farson DA, Hatier C, Hassell J and Bissel Mi. (1987): Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells. Proc Natl Acad Sci USA 84: 136–140.
McNamee H, Ingber D and Schwartz M (submitted): Intersection of growth factor and extracellular matrix-activated signaling pathways.
Michalopoulos G and Pitot HC (1975): Primary Culture of Parenchymal Liver Cells on Collagen Membranes. Exp Cell Res 94: 70–78.
Mochitate K, Pawelek P and Grinnell F (1991): Stress relaxation of contracted collagen gels: disruption of actin filament bundles, release of cell surface fibronectin, and down-regulation of DNA and protein synthesis. Exp Cell Res 193: 198–207.
Mooney D, Hansen L, Farmer S, Vacanti J, Langer R and Ingber D (1992): Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J Cell Physiol 151: 497–505.
Mooney DJ, Langer R, Hansen LK, Vacanti JP and Ingber DE (1992): Induction of hepatocyte differentiation by the extracellular matrix and an RGD-containing synthetic peptide. Proc Mat Res Soc Symp Proc 252: 199–204.
Moser GC, Fallon RJ and Meiss HK (1981): Fluorometric measurements and chromatin condensation patterns of nuclei from 3T3 cells throughout Gl. J Cell Physiol 106: 293–301.
Nicolini C, Belmont AS and Martelli A (1986): Critical nuclear DNA size and distribution associated with S phase initiation. Cell Biophys 8: 103–117.
Opas M (1989): Expression of the differentiated phenotype by epithelial cells in vitro is regulated by both biochemistry and mechanics of the substratum. Dev Biol 131: 281–293.
Pienta KJ, Getzenberg RH and Coffey DS (1991): Cell Structure and DNA Organization. Crit Rev Eukary Gene Express 1: 355–385.
Pienta KJ and Coffey DS (1991): Cellular harmonic information transfer through a tissue tensegrity-matrix system. Med Hypoth 34: 88–95.
Plopper G, Schwartz MA, Chen LB, Lechene C and Ingber DE (1991): Binding of fibronectin induces assembly of a chemical signaling complex on the cell surface. J Cell Biol 115: 130a.
Reid LM (1990): Stem Cell Biology, Hormone/Matrix Synergies and Liver Differentiation. Curr Opin Cell Biol 2: 121–130.
Roberts JM and D’Urso G (1988): An origin unwinding activity regulates initiation of DNA replication during mammalian cell cycle. Science 241: 1486–1489.
Sachs F (1989): Ion channels as mechanical transducers. In: Stein W, Bronner F, eds. Cell Shape: Determinants, Regulation and Regulatory Role. Academic Press: Orlando, pp. 63–92.
Sawada N, Tomomura A, Sattler CA, Sattler GL, Kleinman HK and Pitot HC (1987): Effects of Extracellular Matrix Components on the Growth and Differentiation of Cultured Hepatoctyes. In Vitro Cell Dev Biol 23: 267–273.
Schwartz MA (1992): Transmembrane signalling by integrins. Trends Cell Biol 2: 304–308.
Schwartz MA, Ingber DE, Lawrence M, Springer TA and Lechene C (1991a): Multiple Integrins Share the Ability to Induce Elevation of Intracellular pH. Exp Cell Res 195: 533–535.
Schwartz MA, Lechene C and Ingber DE (1991b): Insoluble Fibronectin Activates the Na+/H+ Antiporter by Clustering and Immobiliving Integrin a5ß1, independent of cell shape. Proc Natl Acad Sci USA 88: 7849–7853.
Sims J, Karp S and Ingber DE (1992): Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal, and nuclear shape. J Cell Sci - in press.
Stoker AW, Streuli CH, Martins-Green M and Bissell MJ (1990): Designer Microenvironments for the Analysis of Cell and Tissue Function. Curr Opin Cell Biol 2: 864–874.
Vacanti JP, Morse M, Saltzman W, Domb A, Perez-Atayde A and Langer R (1988): Selective cell transplantation using bioabsorbable artificial polymers as matrices. J Pediatr Surg 23: 3–9.
Wang N, Butler JP and Ingber DE (submitted): Probing the molecular basis of mechanotransduction across the cell surface and through the cytoskeleton.
Yen A and Pardee AB (1979): Role of nuclear size in cell growth initiation. Science 204: 1315–1317.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Birkhäuser Boston
About this chapter
Cite this chapter
Ingber, D. (1993). Extracellular Matrix, Cellular Mechanics and Tissue Engineering. In: Bell, E. (eds) Tissue Engineering. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4615-8186-4_7
Download citation
DOI: https://doi.org/10.1007/978-1-4615-8186-4_7
Publisher Name: Birkhäuser, Boston, MA
Print ISBN: 978-1-4615-8188-8
Online ISBN: 978-1-4615-8186-4
eBook Packages: Springer Book Archive