Abstract
This chapter will review immunologic and immunohistochemical data concerning the nature and distribution of contractile proteins in endothelial and smooth muscle cells in different segments of the human vascular tree. Evidence will also be presented indicating that the content of actomyosin in endothelial cells of cerebral vessels can increase in hypertension. This data will be reviewed in the light of a body of knowledge gained largely over the past decade concerning the nature of contractile proteins of “non-muscle” cells and their role in cell motility, maintenance of cell shape, secretory processes, movement of subcellular organelles, attachment to matrix, and cell division.
This work was supported by research grants NHLBI 01803 and 18828 and a grant from the Cross Foundation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adelstein, R.S., and Conti, M.A., 1975, Phosphorylation of platelet myosin increases actin-activated myosin ATPase activity. Nature, 256: 597–598.
Adelstein, R.S., Chacko, S., Barylko, B., Scordelis, S.P., and Conti, M.A., 1976, The role of myosin phosphorylation in the regulation of platelet and smooth muscle contractile proteins, in Contractile Systems in Non-Muscle Tissues (ed., S.V. Perry, A. Margreth, and R.S. Adelstein), Elsevier/ North Holland, pp. 153–163.
Adelstein, R.S., and Pollard, T.D., 1978, Platelet contractile proteins, in Progress in Hemostasis and Thrombosis (ed., T.H. Spaet), Grune and Stratton, New York, pp. 37–58.
Adelstein, R.S., Trotter, J.A., Hathaway, D.R., Heinen, R., and Conti, M.A., 1979. Studies on myosin light chain kinase in macrophages, scallops and platelets, in motility in cell function: Proceedings of the First John M, Marshall Symposium in Cell Biology (ed., F.A. Pepe, J.W. Sanger, and V.T. Nachmias) Academic Press, New York, pp. 161–178.
Avrameas, S., 1969, Coupling of enzymes to proteins with glutaralde-hyde. Immunochemistry, 6: 42–55.
Becker, C.G., and Murphy G.E., 1969, Demonstration of contractile protein in endothelium and cells of heart valves, endocardium, intima, arteriosclerotic plaques, and Aschoff bodies of rheumatic heart disease. Am. J. Path., 55: 1–37.
Becker, C.G., and Shustak, S.R., 1972, Contractile protein in endothelial cells: Comparison of cerebral capillaries with those in heart and skeletal muscle and with liver sinusoids. Circulation, 46, Suppl 2: 87.
Becker, C.G., 1972, Demonstration of actomyosin in mesangial cells of the renal glomerulus. Am. J. Path., 66: 97–110.
Becker, C.G., and Hardy, A.M., 1973, Contractile protein in endothelial cells of normotensive and malignant hypertensive states. Circulation, 48, Suppl IV: 44.
Becker, C.G., and Nachman, R.L., 1973, Contractile proteins of endothelial cells, platelets and smooth muscle. Am. J. Path., 71: 1–21.
Becker, C.G., 1976, Contractile and relaxing proteins of smooth muscle and platelets: Their presence in the endothelium. An. NY Acad. Sci., 275:78:86.
Burridge, K., and Bray, D., 1975, Purification and structural analysis of myosins from brain and other non-muscle tissues. J. Molec. Biol., 99: 1–14.
Clarke, M., and Spudich, J.A., 1977, Non-muscle contractile proteins: The role of actin and myosin in cell motility and shape determination. Ann. Rev. Biochem., 46:797:822.
Cohen, J., and Cohen C., 1972, A tropomyosin-like protein from human platelets. J. Molec. Biol., 68: 383–387.
Dabrowska, R., Sherry, J.M.F., Aramatorio, D.K., and Hartshorne, D.J., 1978, Modulator protein as a component of the myosin light chain kinase from chicken gizzard. Biochem., 17: 253–258.
Dabrowska, R., Sherry, J.M.F., and Hartshorne, D.J., 1979, Phosphorylation of myosin: A possible regulatory mechanism in smooth muscle, in Motility in Cell Function, Proceedings of the First John Marshall Symposium in Cell Biology (ed., F.A. Pepe, J.W. Sanger, and V.T. Nachmias), Academic Press, New York, pp. 147–160.
Elzinga, M., Maron, B.J., and Adelstein, R.S., 1975, Human heart and platelet actions are products of different genes. Science, 191: 94–95.
Elzinga, M., and Kollega, J., 1978, Smooth muscle contains “muscle type” actin. Fed. Proc., 37:1694.
Fine, R.E., and Blitz, A.L., 1976, Chemical and functional studies of tropomyosin and troponin C from brain and other tissues in cell motility. Conference on Cell Proliferation, Vol. 3, Book B (ed., R. Goldman, T. Pollard, and J. Rosenbaum), Cold Spring Harbor, N.Y. pp. 785–795.
Gabbiani, G., Badonel, M.C., and Rona, G., 1975, Cytoplasmic contractile apparatus in aortic endothelial cells of hypertensive rats. Lab. Invest., 32: 227–234.
Garrels, J.I., and Gibson, W., 1976, Identification and characterization of multiple forms of actin. Cell, 9: 793–805.
Giacomelli, F., Wiener, J., and Spiro, D., 1970, The cellular pathology of experimental hypertension. V. Increased permeability of cerebral arterial vessels. Am. J. Path., 59: 133–160.
Goldman, R.D., Milsted, A., Schloss, J.A., Starger, J., and Yera, M-J., 1979, Cytoplasmic fibers in mammalian cells: Cytoskel-etal and contractile elements. Ann. Rev. Physiol., 41: 703–722.
Gorschel-Stewart, U., 1980, Biochemistry and immunochemistry of cytoplasmic filamentous structures. European J. Cancer, 16: 2–4.
Henson, P.M., 1977, Immune complex diseases. Cellular mediators and the pathogenesis of inflammatory tissue injury produced by immune complexes, in Bayer Symposium VI, Experimental Models of Chronic Inflammatory Diseases. Springer-Verlag, pp. 94–106.
Joris, I., and Majno, G., 1980, Endothelial and medial injury caused by arterial spasm in the rat. Fed. Proc. 39:4434.
Lowey, S., Silberstein, L., Gauthier, G., and Holt, J.C., 1979, Isolation and distribution of myosin isoenzymes in motility and cell function. Proceedings of the First John M. Marshall Symposium in Cell Biology (ed., F.A. Pepe, J.W. Sanger, and V.T. Nachmias), Academic Press, New York, pp. 53–67.
Majno, G., Shea, S.M., and Levanthal, M., 1969, Endothelial contraction induced by Histamine-type mediators. An electron microscopic study. J. Cell. Biol., 42: 647–672.
Majno, G., Joris, I., 1977, Endothelium. A Review, in The Thrombotic Process in Atherogenesis (ed., A.B. Chandler, Jr.), Plenum Press, New York, pp. 169–243.
McMillan, G.C., 1979, Cardiovascular Diseases, in Smoking and Health, a report of the Surgeon General, DHEW Publication No PHS 79-5066, pp. 4–3 to 4–76.
Moore, A., Jaffe, E.A., Becker, C.G., and Nachman, R.L., 1977, Myosin in cultured human endothelial cells. Br. J. Haematol., 35: 71–79.
Nairn, A.C., and Perry, S.V., 1979, The role of calmodulin in the myosin light-chain kinase system. Biochem. Soc. Trans., 7:966–967
Perry, S.V., Cole. H.A., and Dhoot, G.K., 1979, The regulatory proteins of the I filament and the control of contractile activity in different types of striated muscle, in Motility in Cell Function: Proceedings of the First John M. Marshall Symposium in Cell Biology (ed., F.A. Pepe, J.S. Sanger, and V.T. Nachmias), Academic Press, New York, pp. 129–145.
Pollard, T.D., and Weihing R.R., 1974, Actin, myosin and cell movement. Crit. Rev. Biochem., 2: 1–65.
Reese, T.S., and Karnovsky, M.J., 1967, Fine structural localization of a blood brain barrier to exogenous peroxidase. J. Cell Biol., 34: 207–217.
Robertson, A.L., Jr., and Khairallah, P.H., 1973, Arterial endothelial permeability and vascular disease. The “trap-door” effect. Exp. Mol. Path., 18: 241–260.
Rubenstein, P.A., and Spudich, J.A., 1977, Actin microheterogeneity in chick embryo fibroblasts. Proc. Nat’l. Acad. Sci., 74: 120–123.
Scheinman, J.I., Fish, A.L., and Michael, A.F., 1974, The immuno-histopathology of glomerular antigens. J. Clin. Invest., 54: 1144–1154.
Sherry, J.M.F., Gorecka, A., Aksoy, M.O., Dabrowska, R., and Hartshorn, D.J., 1978, Roles of calcium and phosphorylation in the regulation of activity of gizzard myosin. Biochem., 17: 4411–4418.
Storti, R.V., and Rich, A., 1976, Chick cytoplasmic actin and muscle actin have different structural genes. Proc. Nat’l. Acad. Sci., 73: 2346–2350.
Stossel, T.P., and Hartwig, J.H., 1976, Phagocytosis and the contractile proteins of pulmonary macrophages, in Cell Motility Conference on Cell Proliferation, Vol. 3, Book B (ed., R. Goldman, T. Pollard, and J. Rosenblum). Cold Spring Harbor Lab., Cold Spring Harbor, N.Y. pp. 529–544.
Stossel, T.P., Hartwig, J.H., and Boxer, L.A., 1976, Contractile proteins and the mechanism of phagocytosis, in Contractile Systems in Non-muscle Tissue (ed., S.V. Perry, A. Margreth, and R.S. Adelstein). Elsevier/ North Holland, pp. 323–329.
Walsh, M.P., Vallet, B., Autric, F., and Demaille, J.G., 1979, Purification and characterization of bovine cardiac calmodulin dependent myosin light chain kinase. J Biol. Chem., 254: 12136–12144.
Wang, J.H., and Desal, R., 1976, A brain protein and its effect on the Ca2+ and protein modulator activated cyclic nucleotide phosphodiesterase. Biochem. Biophys. Res. Commun., 72: 926–932.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Plenum Press, New York
About this chapter
Cite this chapter
Becker, C.G. (1981). Contractile Proteins of Vascular Endothelium and Smooth Muscle. In: Schwartz, C.J., Werthessen, N.T., Wolf, S. (eds) Structure and Function of the Circulation. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7927-4_9
Download citation
DOI: https://doi.org/10.1007/978-1-4615-7927-4_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-7929-8
Online ISBN: 978-1-4615-7927-4
eBook Packages: Springer Book Archive