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An Example of Analysis

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Single-Channel Recording

Abstract

This chapter demonstrates some of the computer programs and analysis techniques currently in use in our laboratory. A comparison with the techniques described in Chapters 11 and 12 will show that the methods shown here are not necessarily the most advanced ones. The level of automation is relatively low, so that the process of fitting channel transitions might be termed a computer-aided manual procedure. Our goal has been to make this procedure fairly rapid while still allowing the operator to observe every transition in the current record. The analysis is demonstrated with a recording of acetylcholine receptor currents that shows a variety of kinetic features.

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References

  • Colquhoun, D., and Hawkes, A. G., 1981, On the stochastic properties of single ion channels, Proc. R. Soc. (Lond.) [Biol.] 211:205–235.

    Article  CAS  Google Scholar 

  • Fenwick, A. M., Marty, A., and Neher, E., 1982, Sodium and calcium channels in bovine chromaffin cells,J. Physiol. (Lond.) 331:599–635.

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  • Sakmann, B., 1978, Acetylcholine-induced ionic channels in rat skeletal muscle, Fed. Proc. 37:2654–2659.

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  • Sakmann, B., Patlak, J., and Neher, E., 1980, Single acetylcholine-activated channels show burst-kinetics in presence of desensitizing concentrations of agonist, Nature 286:71–73.

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© 1983 Plenum Press, New York

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Sigworth, F.J. (1983). An Example of Analysis. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7858-1_14

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  • DOI: https://doi.org/10.1007/978-1-4615-7858-1_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-7860-4

  • Online ISBN: 978-1-4615-7858-1

  • eBook Packages: Springer Book Archive

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