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Ribosomes and Translation

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Ribosomes

Part of the book series: Cellular Organelles ((CORG))

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Abstract

By 1940 Albert Claude had succeeded in isolating from animal cells cytoplasmic RNA-containing granules that were smaller than mitochondria. These granules varied from 50 to 200 μ in diameter and later Claude began calling them microsomes. Chemical analyses indicated that Claude’s microsomes were “phospholipid-ribonucleoprotein complexes.”

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References

  • Brachet, J. (1942) La localisation des acides pentosenucleiques dans les tissues animaux et les oeufs d’amphibiens en voie de developement, Arch. Biol. 53:207–257.

    Google Scholar 

  • Casperson, T. (1941) Studien uber den Eiweissumsatz der Zelle, Naturwissenschaften 29:33–43.

    Article  Google Scholar 

  • Chao, F.-C., and Schachman, H. K. (1956) The isolation and characterization of a macromolecular ribonucleoprotein from yeast, Arch. Biochem. Biophys. 61:220–230.

    Article  PubMed  CAS  Google Scholar 

  • Claude, A. (1940) Particulate components of normal and tumor cells, Science 91:77–78.

    Article  PubMed  CAS  Google Scholar 

  • DeVries, J. K., and Zubay, G. (1967) DNA-directed peptide synthesis, II. The synthesis of the α-fragment of the enzyme β-galactosidase, Proc. Natl. Acad. Sci. USA 57:1010–1012.

    Article  PubMed  CAS  Google Scholar 

  • Gierer, A. (1963) Function of aggregated reticulocyte ribosomes in protein synthesis, J. Mol. Biol. 6:148–157.

    Article  PubMed  CAS  Google Scholar 

  • Gold, L. M., and Schweiger, M. (1969) Synthesis of phage-specific α-and β-glucosyl transferases directed by T-even DNA in vitro, Proc. Natl. Acad. Sci. USA 62:892–898.

    Article  PubMed  CAS  Google Scholar 

  • Lamborg, M., and Zamecnik, P. C. (1960) Amino acid incorporation by extracts of E. coli, Biochim. Biophys. Acta. 42:206–211.

    Article  PubMed  CAS  Google Scholar 

  • Littlefield, J. W., and Keller, E. B. (1957) Incorporation of C14 amino acids into ribonucleoprotein particles from the Ehrlich mouse ascites tumor, J. Biol. Chem. 224:13–30.

    PubMed  CAS  Google Scholar 

  • Littlefield, J. W., Keller, E. B., Gross, J., and Zamecnik, P. C. (1955) Studies on cytoplasmic ribonucleoprotein particles from the liver of the rat, J. Biol. Chem. 217:111–123.

    PubMed  CAS  Google Scholar 

  • Martin, K. A., and Miller, O. L. (1983) Polysome structure in sea urchin eggs and embryos: An electron microscopic analysis, Dev. Biol. 98:338–348.

    Article  PubMed  CAS  Google Scholar 

  • Matthaei, H., and Nirenberg, M. W. (1961) The dependence of cell-free protein synthesis in E. coli upon RNA prepared from ribosomes, Biochem. Biophys. Bes. Commun. 4:184–189.

    Google Scholar 

  • McQuillen, K., Roberts, R. B., and Britten, R. J. (1959) Synthesis of nascent protein by ribosomes in E. coli, Proc. Natl. Acad. Sci. USA 45:1437–1447.

    Article  PubMed  CAS  Google Scholar 

  • Nirenberg, M. W., and Matthaei, J. H. (1961) The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polynucleotides, Proc. Natl. Acad. Sci. USA 47:1588–1602.

    Article  PubMed  CAS  Google Scholar 

  • Palade, G. E. (1955) A small particulate component of the cytoplasm, J. Biophys. Biochem. Cytol. 1:59–68.

    Article  PubMed  CAS  Google Scholar 

  • Palade, G. E., and Siekevitz, P. (1956a) Liver microsomes: An integrated morphological and biochemical study. J. Biophys. Biochem. Cytol. 2:171–200.

    Article  PubMed  CAS  Google Scholar 

  • Palade, G. E., and Siekevitz, P. (1956b) Pancreatic microsomes: An integrated morphological and biochemical study, J. Biophys. Biochem. Cytol. 2:671–691.

    Article  PubMed  CAS  Google Scholar 

  • Penman, S., Scherrer, K., Becker, Y., and Darnell, J. (1963) Polyribosomes in normal and poliovirus-in-fected HeLa cells and their relationship to messenger-RNA, Proc. Natl. Acad. Sci. USA 49:654–662.

    Article  PubMed  CAS  Google Scholar 

  • Petermann, M. L., and Hamilton, M. G. (1957) The purification and properties of cytoplasmic ribonucleoprotein from rat liver, J. Biol. Chem. 224:725–736.

    PubMed  CAS  Google Scholar 

  • Roberts, R. B., ed. (1958) Microsomal particles and protein synthesis, First Symposium of the Biophysical Society, at The Massachusetts Institute of Technology, Cambridge, Mass., February 5, 6, and 8, 1958 (London, New York, Paris, and Los Angeles: Pergamon Press).

    Google Scholar 

  • Schachman, H. K., Pardee, A. B., and Stanier, R. Y. (1952) Studies on the macromolecular organization of microbial cells, Arch. Biochem. Biophys. 38:245–260.

    Article  PubMed  CAS  Google Scholar 

  • Schachtschabel, D., and Zillig, W. (1959) Untersuchungen zur Biosynthese der Proteine. I. Uber den Einbau 14C-markierter Aminosäuren ins Protein zellfreier Nucleoproteid-Enzym-Systeme aus E. coli B, Hoppe-Seyler’s Z. Physiol. Chem. 314:262–275.

    Article  PubMed  CAS  Google Scholar 

  • Spirin, A. S. (1990) Ribosome preparation and cell-free protein synthesis, in The Bibosome: Structure, Function, and Evolution (W. E. Hill, A. Dahlberg, R. A. Garrett, P. B. Moore, D. Schlesinger, and J. R. Warner, eds.) pp. 56–70, ASM Press, Washington, DC.

    Google Scholar 

  • Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S. Yu., and Alakhov, and Yu. B. (1988) A continuous cell-free translation system capable of producing polypeptides in high yield, Science 242:1162–1164.

    Article  PubMed  CAS  Google Scholar 

  • Tissieres, A., and Watson, J. D. (1958) Ribonucleoprotein particles from E. coli, Nature 182:778–780.

    Article  PubMed  CAS  Google Scholar 

  • Tissieres, A., Schlessinger, D., and Gros, F. (1960) Amino acid incorporation into proteins by E. coli ribosomes, Proc. Natl. Acad. Sci. USA 46:1450–1463.

    Article  PubMed  CAS  Google Scholar 

  • Ts’o, P. O. P., Bonner, J., and Vinograd, J. (1956) Microsomal nucleoprotein particles from pea seedlings, J. Biophys. Biochem. Cytol. 2:451–465.

    Article  CAS  Google Scholar 

  • Warner, J. R., Knopf, P. M., and Rich, A. (1963) A multiple ribosomal structure in protein synthesis, Proc. Natl. Acad. Sci. USA 49:122–129.

    Article  PubMed  CAS  Google Scholar 

  • Watson, J. D. (1963) The involvement of RNA in the synthesis of proteins, Science 140:17–26.

    Article  PubMed  CAS  Google Scholar 

  • Wettstein, F. O., Staehelin, T, and Noll, H. (1963) Ribosomal aggregate engaged in protein synthesis: Characterization of the ergosome, Nature 197:430–435.

    Article  PubMed  CAS  Google Scholar 

  • Zamecnik, P. C. (1953) Incorporation of radioactivity from D,L-leucine-1-14C into proteins of rat liver homogenates, Fed. Proc. 12:295.

    Google Scholar 

  • Zamecnik, P. C., and Keller, E. B. (1954) Relation between phosphate energy donors and incorporation of labeled amino acids into proteins, J. Biol. Chem. 209:337–354.

    PubMed  CAS  Google Scholar 

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© 1999 Kluwer Academic/Plenum Publishers, New York

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Spirin, A.S. (1999). Ribosomes and Translation. In: Ribosomes. Cellular Organelles. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7817-8_4

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  • DOI: https://doi.org/10.1007/978-1-4615-7817-8_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-46146-0

  • Online ISBN: 978-1-4615-7817-8

  • eBook Packages: Springer Book Archive

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