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Methods for Isolation, Characterization, and Sequence Analysis of Enkephalin Precursors

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Research Methods in Neurochemistry

Abstract

A phenomenon of many CNS-active drugs is that their actions are mediated through interactions at specific receptors.This is true for the opiate narcotics, which exert their effects by interacting with receptors whose endogenous ligands are opioid peptides such as the enkephalins and β-endorphin. The two major research advances that led to this conclusion were the findings that labeled opiate drugs bound stereospecifically and with high affinity to receptor sites in brain membranes and the discovery in 1975 that opiatelike substances existed in brain. These compounds were characterized as pentapeptides (the enkephalins) and later as the 31-amino-acid peptide β-endorphin. The emphasis of this introduction is to provide background material in order to provide insight into the studies conducted on the biosynthesis of the opioid peptides. In the course of this review, recently developed methods are discussed that were necessary in order to deduce the biosynthesis of these molecules.

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Authors and Affiliations

  1. Department of Biopolymer Research, Roche Research Center, Hoffmann-La Roche Inc., Nutley, New Jersey, 07110, USA

    Alvin S. Stern

  2. Department of Biochemistry, University of Wyoming, Laramie, Wyoming, 82071, USA

    Randolph V. Lewis

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  1. Alvin S. Stern
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Editors and Affiliations

  1. Center for Neurochemistry, Rockland Research Institute, Wards Island, New York, New York, USA

    Neville Marks

  2. Department of Biochemistry, Institute of Psychiatry, University of London, London, Great Britain

    Richard Rodnight

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Stern, A.S., Lewis, R.V. (1985). Methods for Isolation, Characterization, and Sequence Analysis of Enkephalin Precursors. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7760-7_5

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  • DOI: https://doi.org/10.1007/978-1-4615-7760-7_5

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