Abstract
Synaptic vesicles are the small organelles approximately 30–100 nm in diameter seen characteristically aggregated in the preterminal ending at most synapses. It was proposed, originally by Del Castillo and Katz (1955), de Robertis and Bennett (1955), and Palay (1956), that the vesicles store the quanta of transmitter prior to their release. They are, on this view, the morphological counterparts of the quanta. It has also been proposed that the vesicles are involved in the actual processes of release of transmitter, reverse micropinocytosis or exocytosis of the vesicle contents being the most favored mechanism (for review, see Hubbard, 1970). Both hypotheses are accessible to biochemical investigation provided that methods can be devised for the isolation and chemical study of synaptic vesicles. This chapter describes methods currently available and offers some comments on their applicability and interpretation.
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Marchbanks, R.M. (1974). Isolation and Study of Synaptic Vesicles. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7751-5_4
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DOI: https://doi.org/10.1007/978-1-4615-7751-5_4
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