Abstract
With the advent of various types of chromatography, a wide choice of methods is now available for the resolution and isolation of amino acids in complex mixtures. For quantitative analysis, paper chromatography and ion-exchange chromatography are the methods of choice. One-dimensional rather than two-dimensional paper chromatography is preferred as a routine procedure, samples being pretreated with ion-exchange resins to obtain groups of amino acids. Reliable methods for the isolation of the amino acids in the brain after paper chromatography have been published (Gaitonde, 1961; Gaitonde et al., 1965; Robinson and Williams, 1965).
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References
Benson, J. V., Gordon, M. J., and Patterson, J. A. (1967) Anal. Biochem. 18, 228.
Berl, S., Lajtha, A., and Waelsch, H. (1961) J. Neurochem. 7, 186.
Bray, G. A. (1960) Anal. Biochem. 1, 279.
Dickinson, J. C., Rosenblum, H., and Hamilton, P. B. (1965) Pediatrics 36, 2.
Doležalova, V., and Brada, Z. (1964) Clin. Chim. Acta 10, 34.
Evans, G. M., Thompson, J., and Gaitonde M. K. (1972) Unpublished work.
Fischer, F. G., and Bohn, H. (1955) Z. Physiol. Chem. 302, 278.
Gaitonde, M. K. (1961) J. Neurochem. 8, 234.
Gaitonde, M. K., and Arnfred, T. (1971) J. Neurochem. 18, 1971.
Gaitonde, M. K., and Kelly, R. (1972) J. Neurochem. 19, 377.
Gaitonde, M. K., and Nixey, R. W. K. (1972) Anal. Biochem. 50, 416.
Gaitonde, M. K., Dahl, D. R., and Elliott, K. A. C. (1965) Biochem. J. 94, 345.
Hall, T. C., and Cocking, E. C. (1965) Biochem. J. 96, 626.
Hamilton, P. B. (1945) J. Biol. Chem. 158, 375.
Hamilton, P. B. (1958) Anal. Chem. 30, 914.
Hamilton, P. B. (1962) Ann. N.Y. Acad. Sci. 102(i), 55.
Hamilton, P. B. (1963) Anal. Chem. 35, 2055.
Hamilton, P. B., and Van Slyke, D. D. (1943) J. Biol. Chem. 150, 231.
Hirs, C. H. W., Moore, S., and Stein, W. H. (1954) J. Am. Chem. Soc. 76, 6063.
Lajtha, A., Furst, S., Gerstein, A., and Waelsch, H. (1957) J. Neurochem. 1, 289.
MacFadyen, D. A. (1950) J. Biol. Chem. 186, 1.
MacLennan, D. H., Beevers, H., and Harley, J. L. (1963) Biochem. J. 89, 316.
Matheson, A. T., Tigane, E., and Hanes, C. S. (1961) Can. J. Biochem Physiol. 39, 417.
Meyer, H. (1957) Biochem. J. 67, 333.
Minard, F. N., and Mushahwar, I. K. (1966a) Life Sci. 5, 1409.
Minard, F. N., and Mushahwar, I. K. (1966b) J. Neurochem. 13, 1.
Moore, S., and Stein, W. H. (1948) J. Biol Chem. 176, 367.
Moore, S., and Stein, W. H. (1954) J. Biol. Chem. 211, 907.
Moore, S., Spackman, D. H., and Stein, W. (1958) Anal. Chem. 30, 1185.
Oreskes, I., Cantor, F., and Kupfer, S. (1965) Anal. Chem. 37, 1720.
Perry, T. L., Stedman, D., and Hansen, S. (1968) J. Chromatog. 38, 460.
Porcellati, G., and Thompson, R. H. S. (1957) J. Neurochem. 1, 340.
Rajagopalan, T. G., Moore, S., and Stein, W. H. (1966) J. Biol. Chem. 241, 4940.
Roberts, E. (1962) In Amino Acid Pools (J. T. Holden, ed.), Elsevier, Amsterdam, p. 517.
Roberts, E., and Frankel, S. (1950) J. Biol. Chem. 187, 55.
Robinson, N., and Williams, C. B. (1965) Clin. Chim. Acta 12, 311.
Rosen, H. (1957) Arch. Biochem. Biophys. 67, 10.
Saifer, A. (1971) Anal. Biochem. 40, 412.
Spackman, D. H., Stein, W. H., and Moore, S. (1958) Anal. Chem. 30, 1190.
Stein, W. H., and Moore, S. (1954) J. Biol. Chem. 211, 915.
Tallan, H. H., Moore, S., and Stein, W. H. (1956) J. Biol. Chem. 219, 257.
Tallan, H. H. (1962) In Amino Acid Pools (J. T. Holden, ed.), Elsevier, Amsterdam, p. 517.
Tallan, H. H., Moore, S., and Stein, W. (1954) J. Biol. Chem. 211, 927.
Turner, J. C. (1968) Internat. J. Appl. Radiat. Isotopes 19, 557.
Vega, A., and Nunn, P. B. (1969) Anal. Biochem. 32, 446.
Yemm, E. W., and Cocking, E. C. (1955) Analyst (Lond.) 80, 209.
Yoshino, Y., and Elliott, K. A. C. (1970) Can. J. Biochem. 48, 228.
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© 1974 Plenum Press, New York
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Gaitonde, M.K. (1974). Methods for the Isolation and Determination of Glutamate, Glutamine, Aspartate, and γ-Aminobutyrate in Brain. In: Marks, N., Rodnight, R. (eds) Research Methods in Neurochemistry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7751-5_13
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DOI: https://doi.org/10.1007/978-1-4615-7751-5_13
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