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A RP-HPLC Method for the Measurement of Guanine, Other Purine Bases and Nucleosides

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Purine and Pyrimidine Metabolism in Man VII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 309B))

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Abstract

A method for the analysis of purine bases and their nucleosides by RP-HPLC has been developed using a procedure similar to that reported in Reference 1. A chromatogram of standard compounds is shown in Figure 1. The method has been employed for the evaluation of purine metabolites in plasma from subjects admitted to the neonatal intensive care unit of the University of Rome (A) and for the study of allopurinol metabolism in human heart (B).

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Author information

Authors and Affiliations

  1. Institute of Pediatrics, University of Rome, Italy

    C. P. Quaratino, E. Messina, P. Bertuglia, G. Spoto, G. Sciarra, G. Bucci & A. Giacomello

  2. Institutes of Biochemical Sciences and Human Physiopathology, University of Chieti, and Hospital of Castel Di Sangro-Italy, Italy

    C. P. Quaratino, E. Messina, P. Bertuglia, G. Spoto, G. Sciarra, G. Bucci & A. Giacomello

Authors
  1. C. P. Quaratino
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  2. E. Messina
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  3. P. Bertuglia
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  4. G. Spoto
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  5. G. Sciarra
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  6. G. Bucci
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  7. A. Giacomello
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Editor information

Editors and Affiliations

  1. Institute of Child Health, London, UK

    R. Angus Harkness

  2. Burroughs Wellcome Co., Research Triangle Park, North Carolina, USA

    Gertrude B. Elion

  3. Universität München, München, Germany

    Nepomuk Zöllner

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© 1991 Plenum Press, New York

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Quaratino, C.P. et al. (1991). A RP-HPLC Method for the Measurement of Guanine, Other Purine Bases and Nucleosides. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_9

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  • DOI: https://doi.org/10.1007/978-1-4615-7703-4_9

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-7705-8

  • Online ISBN: 978-1-4615-7703-4

  • eBook Packages: Springer Book Archive

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