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Automated Quantitative Analysis for Orotidine and Uridine/Thymine in Urine by High-Performance Liquid Chromatography with Column Switching

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 309B)

Abstract

The measurement of urinary orotidine, uridine and thymine which are intermediates in pyrimidine biosynthesis, is important for screening and diag-nosis inborn ergors of metabolism, such as urea cyclel and pyrimidine metabo-lism disorders 2 , as well as the measurement of urinary orotic acid and uracil l,3. Several high-performance liquid chromatography (HPLC) methods have been reported by a number of researchers. However, these HPLC methods for uridine and thymine analysis require rather complicated sample preparation in order to obtain accurate results 1. Although, a simple method for measuring orotidine and orotic acid were recently reported 4, it is not capable of evaluating near-normal levels of orotidine.

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References

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© 1991 Plenum Press, New York

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Kidouchi, K., Nakamura, C., Katoh, T., Kibe, T., Ohba, S., Wada, Y. (1991). Automated Quantitative Analysis for Orotidine and Uridine/Thymine in Urine by High-Performance Liquid Chromatography with Column Switching. In: Harkness, R.A., Elion, G.B., Zöllner, N. (eds) Purine and Pyrimidine Metabolism in Man VII. Advances in Experimental Medicine and Biology, vol 309B. Springer, New York, NY. https://doi.org/10.1007/978-1-4615-7703-4_7

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  • DOI: https://doi.org/10.1007/978-1-4615-7703-4_7

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4615-7705-8

  • Online ISBN: 978-1-4615-7703-4

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